Construction And Mechanism Of Molecular Detection System For Cefepime And Sotalol Hydrochloride

In recent years,development of a rapid,green and sensitive drug detection analysis technology has been widely focused by many scholars.Among them,the two commonly used probes are the fluorescent probes based on organic small molecule of fluorescent dyes and their derivatives and the phosphorous probes based on nanomaterials.Quantum dots,magnetic carbon nanotubes and gold nanoparticles are the main nano-phosphorous probes.Semiconductor quantum dots(QDS)have better physical and chemical properties than traditional organic molecular materials due to their special structure,and show a wealth of functions.As a result,more and more fields,such as computer and microelectronics,environment and energy,aerospace and defense,and biosensors and drug detection,present attractive prospects.Human research on biological molecules from gene level will not only help to understand the mechanism and principle of some disease,provides a reference theoretical basis for the pathogenesis of various diseases,but also enables design of appropriate molecular assemblies probe can quantitatively detect the content of drug molecules in the body,which provides the necessary scientific basis and theoretical guidance for the determination and use of drug doses in clinical medicine.With the continuous development of nano-detection technology,the detection of drug molecules by nano-phosphorescent probes is also more accurate,which plays an indispensable role in people’s actual production and life.In this paper,water phase synthesis method was used to prepare 3-mercaptopropionic acid(MPA)coated Mn doped ZnS quantum dots(Mn: ZnS QDs)with room temperature phosphorescence(RTP).The molecular detection systems of cefepime and sotalol hydrochloride were constructed respectively.The paper is divided into four parts,and the detailed research contents are as follows:(1)Based on the RTP characteristics of MPA coated Mn: ZnS QDs,the MPA coated Mn:ZnS QDs was conjugated with Trypsin by 1-(3-dimethylaminopropyl)-3-ethylcarbinimide dihydrochloride(EDC)and N-hydroxysuccinimide(NHS)to synthesize quantum dot-trypsin(QDs-Try)nanocomposite phosphorescent probes.A new detection method of Cefepime(CFPM)was established.When Cefepime was added to the system,the room temperature phosphorescence of the quantum dot-trypsin(QDs-Try)nanocomposite phosphorescent probe were quenching regularly,and detection of CFPM could be realized.The linear detection range of this method was 0.1 ～ 36 μmol / L,and the detection limit was 0.03 μmol / L.In addition,the detection was carried out in the phosphorescent mode to eliminate the background interference in the fluorescence mode,and the detection of CFPM in human urine samples was successfully realized,with the spiked recovery is 97.2% ～ 101.1%.(2)Based on the phosphorescence quenching effect between sotalol hydrochloride and Mn-doped ZnS quantum dot / p-benzoquinone complex(Mn: ZnS QDs / BQ),a new and simple method for determination of sotalol hydrochloride was established.This method effectively utilizes the physical and optical properties of Mn: ZnS QDs and the specific recognition of p-benzoquinone for sotalol hydrochloride.The various spectral parameters of the system are studied in detail,illuminates the Mn: ZnS QDs / BQ nano composite administered with sotalol hydrochloride mechanism.The composite phosphorescent probe was successfully applied to the determination of sotalol hydrochloride in human urine.the detection limit is 0.022 μmol / L,the spiked recovery is 95.6% ～ 98.2%,and the result is satisfactory.In conclusion,this paper based on the room temperature phosphorescence characteristics of MPA-coated Mn: ZnS QDs,and fully investigated the structure and chemical properties of the target drug molecules cefepime and sotalol hydrochloride.the QDs-Try nano composite phosphorescence probe and Mn: ZnS QDs / BQ nanocomplexes were established used for detection of cefepime and sotalol hydrochloride,It was confirmed through experiments that the experimental method can be used for the detection of two kinds of drug molecules,which provides a new idea for the determination of active molecules of drugs.