Chlorogenic Acid Improves Memory Impairment And Cognitive Impairment In Rats With Early Alzheimer’s Disease Through Wnt Signaling Pathway

ObjectiveTo explore whether chlorogenic acid can improve memory impairment and cognitive impairment in Alzheimer’s disease rats through wnt signaling pathway.MethodsForty-five male rats were selected to prevent accidental loss from affecting the progress of the experiment.The rats were randomly divided into Ctrl group,AD group,CHA100 group,and CHA150 group.Among them,CHA100 and CHA150 groups were given chlorogenic acid gavage treatment according to their respective group doses one week before modeling.Three days after the completion of modeling,except for the Ctrl group,they were continuously administered for 6 W,and passed the Morris water maze experiment and open field experiment.Analyze the changes in rat behavioral data to detect the cognitive level and memory ability of rats;immunohistochemistry(Immunohistochemistry,IHC)to observe the levels of inflammatory factors in the hippocampus of rats;Wsten blot to detect whether the wnt signaling pathway is activated and the degree of GFAP activation;HE,Nissl staining was used to observe the morphology of cells in the hippocampus of rats.ResultsIn the results of the water maze experiment,compared with the results of the Ctrl group,the rats in the AD group had longer escape latency,longer swimming distances,and shorter activity time in the target quadrant.Compared with the AD group,the CHA100 group had shorter escape latency.The swimming distance becomes shorter and the activity time in the target quadrant becomes longer.Compared with the AD group,the CHA150 group has shorter escape latency and shorter swimming distance,but the change is not as obvious as that of the CHA100 group.According to the statistical analysis result P<0.05,the difference is statistically significant Significance;in the results of the open field experiment,compared with the results of the Ctrl group,the AD group began to move toward the center of the experiment box,and the activity trajectory was chaotic;compared with the model group,the activity trajectory of the CHA100 group was more inclined to the surrounding;the activity trajectory of the CHA150 group was also It began to lean towards four weeks,but not as good as the CHA100 group.In IHC,the inflammatory factors in the hippocampus of the AD group were significantly increased compared with the Ctrl group,the inflammatory factor expression in the CHA100 group was reduced,and the inflammatory factor expression in the CHA150 group was not significantly reduced.According to the statistical analysis results,P<0.05,The difference is statistically significant.The results of HE staining showed that the hippocampal cells in the AD group shrank and deformed compared with the Ctrl group,and the cell morphology changed.After CHA treatment,the hippocampal cells in the CHA100 group improved,and the cell morphology tended to be normal;the hippocampal cells in the CHA150 group shrank The recovery of the condition was not obvious,and the cell morphology was not significantly improved;Nissl staining results showed that the AD group had less Nissl bodies compared with the Ctrl group.After CHA treatment,the increase of Nissl bodies in the CHA100 group and the less obvious increase of Nissl bodies in the CHA150 group;WB The results showed that the expression levels of GSK-3β,GFAP,and Tau protein increased in the AD group.After treatment,the expression level of the CHA100 group decreased,and the expression level of the CHA150 group decreased,but the therapeutic effect was not as good as that of the CHA100 group;DVL2,β-catenin in AD The expression level in the CHA100 group decreased,the expression level in the CHA100 group increased,and the increase in the CHA150 group expression level was not obvious.According to statistical analysis,P<0.05,the change was statistically significant.ConclusionChlorogenic acid can improve the memory impairment of early Alzheimer’s rats through the wnt signaling pathway.After AD rats took chlorogenic acid,it showed that the wnt pathway was activated,which proved that chlorogenic acid may have a therapeutic effect on Alzheimer’s rats through the wnt signal pathway and may inhibit the development of the disease.