The Expression Of Serum S100B And MMP-9 In Patients With Post-traumatic Late Onset Epilepsy And Research Of Related Factors

Background: Post-traumatic late-onset epilepsy is characterized by high morbidity,high mortality risk,difficult treatment and poor treatment effect,which has attracted extensive clinical attention.Evidence has shown that some biomarkers are closely related to epileptic seizures after brain trauma.However,there are few biomarkers of late-onset epilepsy post-traumatic at home and abroad.Objective: This study intends to determine the levels of serum S100 B and MMP-9in patients with post-traumatic late-onset epilepsy.At the same time,to investigate the relationship between the expression of serum S100 B and MMP-9 in patients with post-traumatic late-onset epilepsy and the related factors.Which provides possible biomarkers and new diagnosis and treatment ideas for the clinical prevent ion and treatment of post-traumatic late-onset epilepsy.Methods: To collect patients were enrolled.to the Department of Neurology and Neurosurgery,inpatients and outpatients,First Affiliated Hospital of Dali University.from Jun 2019 to December 2020.45 patients with post-traumatic late-onset epilepsy were enrolled as the experimental group,and 42 patients with brain trauma but without epilepsy were enrolled as brain trauma control group.41 patients with epilepsy of no definite etiology were enrolled as epilepsy controls.34 patients who were matched with the age and sex of the experimental group were enrolled as normal controls,including baseline data and clinical data.Fasting venous blood was collected from all subjects and serum S100 B and MMP-9 protein contents were determined by enzyme-linked immunosorbent assay(ELISA).Baseline data,the expression levels of S100 B and MMP-9 protein in each group were statistically analyzed.Multiple linear regression analysis was performed on the factors impact the expression of serum S100 B and MMP-9 protein in patients with post-traumatic late-onset epilepsy,to explore the expression of serum S100 B and MMP-9 protein and related factors.Results:1.Incidence rate of post-traumatic late-onset epilepsy: A total of 87 cerebral trauma patients were collected in this study,45 patients were posttraumatic late-onset epilepsy,and the incidence rate of post-traumatic lateonset epilepsy was about 52%.2.The results of enzyme-linked immunosorbent assay(ELISA)showed that the expression of S100 B and MMP-9 protein could be detected in the serum of all subjects.LSD-T test results showed that: The levels of serum S100 B and MMP-9 in the experimental group were higher than those in the brain trauma group and the normal control group,and the difference was statistically significant(P<0.05).The level of serum S100 B in the experimental group was higher than that in the epilepsy group,and the difference was not statistically significant(P>0.05).The level of serum S100 B in epilepsy group was higher than that in brain trauma group,and the difference was statistically significant(P<0.05).The level of serum MMP-9 in the experimental group was higher than that of the brain trauma group and the epilepsy group,and the difference was statistically significant(P<0.05).The level of serum MMP-9 in the brain trauma group was higher than that in the epilepsy group,and the difference was not statistically significant(P>0.05).3.Univariate analysis results of the exp erimental group showed that the type of brain trauma,type of seizure and severity of brain trauma were related to the increase of serum S100 B level in the experimental group(P<0.05),and the type of seizure and severity of brain trauma were related to the serum MMP-9 level in the experimental group(P<0.05).4.The linear correlation analysis results of the experimental group showed that the level of serum S100 B was correlated with the duration of epilepsy,frequency of seizures,number of seizures and duration times of the seizure;The level of serum MMP-9 was correlated with WBC and CRP.5.Univariate analysis and linear correlation analysis were used to select statistically significant related variables for multiple linear regression analysis.The results showed that the duration of epilepsy,the number and frequency of seizures were linearly correlated with the level of serum S100 B in the experimental group;The severity of brain trauma,type of seizure and CRP were linearly correlated with the level of serum MMP-9 in the experimental group.Conclusion:1.Th e post-traumatic late-onset epilepsy has high incidence,and the types of epileptic seizures are mainly focal seizures.There are differences in serum S100 B and MMP-9 levels between different types of epileptic seizures.2.The level of serum S100 B was significantly higher than those in the brain trauma group and normal control group.The level of serum MMP-9 was significantly increased compared with the control group.3.There were differences in the levels of serum S100 B levels among different types of brain trauma in the experimental group,but no significant differences in the level of serum MMP-9 among different types of brain trauma.4.The duration of epilepsy,frequency of seizures and number of seizures affect the expression of serum S100 B protein in patients with posttraumatic late-onset epilepsy;The severity of brain trauma,type of seizure and CRP affect the expression of serum MMP-9 protein in patients with Lateonset epilepsy with post-traumatic.