| Microfluidic Paper-Based Analytical Device(μPad)is a new microfluidic chip technology developed in recent ten years.Compared with traditional materials such as silicon,glass and polymers,paper has the advantages of low cost,simple manufacture,easy-to-use and flexible transportation,and paper-based microfluidic chip can combine the advantages of strip paper test with the analysis and detection function in conventional laboratory.Therefore,this analytical device is called a lab on paper,also known as a microfluidic paper analytical device,and as a disposable analytical device has a great application prospect in biochemical detection,disease diagnosis,environmental monitoring and other fields.Up to now,optical detection and electrochemical detection are commonly used in paper chip analysis.However,these detection methods need to be easy to be labelled for the colorimetric detection,or have the characteristics of electrochemical activity,and these detection methods are difficult to integrate all the unit operations directly on the paper chip,which greatly limits the miniaturization and portable applications of μPAD.Because enzymatic biofuel cell(enzymatic biofuel cell,EBFC)can under the right conditions convert chemical energy into electrical energy,it does not require an external power supply,and do not rely on the traditional three electrode system.This charcteristics let it be easily combined with μPAD to achieve the real-time and stable signal of the sensor the developed paper chip analysis device(EBFC-μPAD)integrated with biofuel cells.Although it represents an important progress in this field,it is limited by the use of paper chips based on origami or pop-up structure,and it is difficult to meet the requirements of EBFC-μPADs.The flexibility of fluid flow is required.Therefore,it is still a challenge to effectively integrate the unit operations such as flexible fluid manipulation,reaction,identification sensing and detection into the paper chip analysis device.On the other hand,thrombin detection is of great significance for the early diagnosis,course monitoring and pathological study of related diseases.The traditional method of thrombin detection is enzyme-linked immunosorbent assay(ELISA),but it has the limitations of low antibody affinity,poor chemical stability,complex preparation and high cost.Therefore,it is an urgent problem to develop a sensitive,rapid,low-cost and convenient method to detect thrombin in the field.Aiming at the above problems,this paper designs and constructs a self-powered Rotating paper chip analysis device(SPR-μPAD).On this device,flexible manipulation,reaction,recognition and sensing of fluid could be successfully to obtain sensitive,rapid,low-cost and miniaturized thrombin detection.The thesis includes the following two parts:The first chapter is the introduction.Firstly,the paper chip analysis device and the significance of thrombin detection are introduced.Then the paper chip analysis device and its research progress in thrombin detection are introduced.Finally,the scientific problems to be solved and the research ideas of this paper are put forward.In the second chapter,a SPR-μPAD is designed and constructed to realize integration of multiple unit operations and sensitive,fast,low-cost and miniaturized μPAD detection.The device is integrated with movable microvalve,self-powered identification sensor,super capacitor,real-time electrical signal output and other units,which is easy to realize flexible fluid control,reaction,recognition and sensing,real-time digital display and sensitive,fast,low cost and miniaturized μPAD detection.On this basis,a new method of high selectivity,high sensitivity,fast and suitable for portable thrombin detection was developed by using the constructed SPR-μPAD,and the effectiveness and operability of SPR-μPAD and this method was verified by standard recovery experiment.Thesemethod will provide a new way for thrombin detection and related disease diagnosis,real-time monitoring of disease course and pathological research. |
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