Study On CO-Metabolites Of Intestinal Flora In Atopic Dermatitis With Heart Fire And Spleen Deficiency

ObjectiveTo explore the co-metabolites of intestinal microflora in patients with atopic dermatitis of heart fire and spleen deficiency type by non-targeting combined with extensive targeted metabonomics detection technology,and to analyze the KEGG pathway annotation and disease association of the significantly different metabolites.Methods20 patients with atopic dermatitis of heart fire and spleen deficiency type and 20 healthy volunteers were included.The collected fecal samples were detected by metabonomics to obtain all metabolites and their relative contents.Principal Component Analysis and Osc Partial Least Squares-Discriminant Analysis were used to analyze the metabolite data to explore the difference between AD experimental group and healthy control group.Based on the results of OPLS-DA analysis,the screening criteria were set to screen out the significantly different metabolites of AD patients with heart fire and spleen deficiency,and Volcano Plot,Heat Map and Violin picture were drawn.KEGG database was used for annotation and functional enrichment analysis of significant difference metabolites in AD patients with heart fire and spleen deficiency,and HMDB database was used for annotation and disease association analysis of significant difference metabolites.Results1.A total of 553 metabolites were detected by non-targeted metabonomics.While,807 metabolites were detected by mass spectrometry.2.PCA showed that there was no significant difference in fecal metabolites between AD experimental group and healthy control group.The OPLS-DA showed that the separation trend between AD experimental group and healthy control group was obvious,and the difference of metabolites between the two groups was obvious.However,the data in the two groups were not concentrated,and there were some differences in the metabolites of the samples in the two groups.3.Based on the results of OPLS-DA,77 differentially expressed metabolites were screened out,among which 39 differentially expressed metabolites were up-regulated.The top five differentially expressed metabolites were LysoPC 22:5(2n isomer2),LysoPC 22:5(2n isomer1),Tryptamine,Indoleacetaldehyde,Phenethylamine.There were 38 differentially expressed metabolites,and the top five differentially expressed metabolites were 18βGlycyrrhetinic acid,Aldosterone,4-Hydroxyretinoic Acid,(5-L-Glutamyl)-L-Amino Acid,Glycyrrhizinate.4.By annotating the significant difference metabolites of AD patients with heart fire and spleen deficiency type,the annotating results are mainly divided into five categories:organic system,metabolism,human diseases,environmental information processing and cell process.In the enrichment results of KEGG pathway,bile secret,aldosterone-regulated sodium reabsorption,aldosterone synthesis and secret were relatively significant.5.Digestive system diseases are the most closely related among the metabolic products related diseases,mainly including colorectal cancer,ulcerative colitis,eosinophilic esophagitis and Crohn’s disease.Conclusion1.The significant co-metabolites of intestinal microflora in AD patients with heart fire and spleen deficiency screened by multivariate statistical analysis still need further specific research.2.The KEGG pathway with significantly different metabolite enrichment is still less studied,which needs further basic research on the pathogenesis of AD.3.At present,there are few studies on AD comorbidity in China.We should include AD comorbidity into chronic disease management and evaluation of AD patients,and try to improve treatment and coping decisions to prevent the occurrence and development of AD comorbidity.