| Objective:This study mainly analyzed the balance of helper T lymphocyte 17(Th 17),regulatory T cell(Treg)and related factors in patients with chronic hepatitis B,and explored the expression of interleukin-6/signal transducer and activator of transcription 3(IL-6/STAT3)and interleukin-2/signal transducer and activator of transcription 5(IL-2/STAT5)in peripheral blood and their effects on Th17/Treg balance in patients with chronic hepatitis B.Methods:In this study,95 patients with chronic hepatitis B who came to the Department of infection of the First Affiliated Hospital of Kunming Medical University from July 2019 to January 2020 were selected as the research objects,and 15 healthy people who came to our hospital during the same period were selected as the healthy control group.Flow cytometer(FCM)was used to detect the frequency of Th17 cells and Treg cells in peripheral blood;Serum IL-2,IL-6,IL-10,IL-17,TGF-P and the concentration of HBeAg were detected by enzyme linked immunosorbent assay(ELISA);The protein contents of STAT3 and STAT5 were detected by Western blot;Serum HBV-DNA load was measured by quantitative real-time PCR;The levels of alt,AST and TBIL were detected by automatic biochemical analyzer.All data were analyzed by SPSS 24.0.Results:1.The levels of Th17,Treg,Th17/Treg,IL-2,IL-6,IL-17 and IL-10 in patients with chronic hepatitis B are significantly higher than those in normal controls(all P<0.05).2.Compared with HBeAg negative patients,Thl7,Th17/Treg and IL-6 and are significantly increased in HBeAg positive patients(all P<0.05);However,there is no significant difference in the levels of Treg,IL-2,IL-10,IL-17 and TGF-β(all P>0.05).Compared with untreated patients,Th17,Treg,Th17/Treg,IL-2,IL-17 and IL-10 in the antiviral treatment group are significantly lower(all P<0.05);but IL-6 and TGF-βthere are no significant difference between the two groups(P>0.05);The imbalance of Th17/Treg is positively correlated with HBV-DNA level(P<0.05).2.The expression of STAT3 and STAT5 protein in CHB patients is significantly higher than that in normal controls(all P<0.001).In patients with chronic hepatitis B,STAT3 protein expression was positively correlated with IL-6,IL-17,Th17 and Th17/Treg(all P<0.05),and negatively correlated with IL-2,and TGF-β(all P<0.05);STAT5 protein expression is positively correlated with Treg cells,but negatively correlated with IL-10(all P<0.05).Conclusion(s):1.There is a significant imbalance of Th17/Treg in patients with chronic hepatitis B.2.The imbalance of Th17/Treg is positively correlated with the level of virus replication.Antiviral therapy and disappearance of e antigen can improve the imbalance of Th17/Treg.3.The increased expression of IL-6/STAT3 and IL-2/STAT5 protein in patients with chronic hepatitis B was positively correlated with the imbalance of Thl7/Treg cells,suggesting that IL-6/STAT3 and IL-2/STAT5 may participate in the formation of Th17/Treg cell imbalance in patients with chronic hepatitis B. |
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