| Objective:Cervical cancer is the most common malignant tumor in female genitalia,which has a serious impact on women’s health.The persistent infection of HR-HPV is the main reason for the occurrence of cervical cancer.The occurrence and development of cervical cancer is a complex process of multi-factor participation and multi-stage control.Small endogenous non-coding RNAs(microRNAs and miRNAs)regulate the post-transcriptional translation of protein-coding genes.MicroRNAs play an important role in the development and progression of cancer and are used as molecular markers for cancer diagnosis,potential therapeutic targets and prognostic evaluation.In this study,clinical cervical tissue samples were collected,and microRNA-1246,RORA,and NF-κB in human cervical tissue were detected by RT-PCR.To analyze the relationship between the expression and clinical data,and to explore the role of the three in the occurrence and development of cervical cancer.The purpose of this study is to provide a basis for further study on whether miR-1246 regulates NF-κB signaling pathway through RORA in cervical cancer.Methods:1、From January 2020 to August 2020,52 cases of cervical cancer tissue were collected from the gynecology department of the Affiliated Hospital of Yunnan University,and 25 cases of normal cervical tissue were collected from the patients who received surgical treatment for benign lesions such as uterine fibroids,adenomyosis and uterine prolapses.RNA extraction and reverse transcription were performed.2、The downstream target gene RORA of miR-1426 was screened by TargetScan7.2,TarBasev8.8,miRBase and miRWalk databases;3、The expression levels of miR-1246,RORA and NF-κB in cervical cancer and normal cervical tissue were examined by RT-RCR method,and the relationship between miR-1246,RORA and NF-κB and clinicopathological indicators such as age,FIGO stage,tumor size and lymph node metastasis was analyzed.Results:1、The relative expression level of miR-1246 in 52 cervical cancer tissues was 1.64±0.23,and that in 25 normal cervical tissues was 1.00±0.16.The relative expression level of miR-1246 in cervical cancer tissues was significantly higher than that in normal cervical tissues(P<0.05).The expression level of miR-1246 in stage Ⅰ and Ⅱ cervical cancer tissues was lower than that in normal cervical tissue,while the expression level of stage Ⅲ and Ⅳ was higher than that in normal cervical tissue(P<0.05).2、The relative expression level of RORA in 52 cervical cancer tissues was 0.41 ±0.09,and that in 25 normal cervical tissues was 1.00±0.23.The relative expression level of RORA in cervical cancer tissues was significantly lower than that in normal cervical tissues(P<0.05).The expression level of RORA was higher in stage Ⅰ and Ⅱ cervical cancer tissues than in normal cervical tissue,and lower in stage Ⅲ and Ⅳcervical cancer tissues(P<0.05).3、The relative expression level of NF-κB was 1.54±0.33 in the whole stage of 52 cases of cervical cancer.The relative expression level of NF-κB in 25 normal cervical tissues was 1.00±0.17,and the relative expression level of NF-κB in cervical tissues was significantly higher than that in normal cervical tissues(P<0.05).The expression level of NF-κB in stage Ⅰcervical cancer tissue was lower than that in normal cervical tissue,while the expression levels of Ⅱ,Ⅲ and Ⅳ were higher than that in normal cervical tissue(P<0.05).4、In cervical cancer tissues,miR-1246 was negatively correlated with the expression of RORA,and the correlation coefficient R value was-0.517(P<0.01).The expression of miR-1426 was positively correlated with NF-κB,and the correlation coefficient r value was 0.975(P<0.01).There was no significant association between RORA and NF-κB expression.5、The expression levels of miR-1246,RORA and NF-κB were correlated with FIGO stage of cervical cancer,and the expression levels of miR-1246 and NF-κB increased gradually with the stage progression(P<0.05).The expression of RORA decreased gradually with the increase of FIGO stage(P<0.05).The expression levels of miR-1246,RORA and NF-κB were not correlated with age,tumor size,pathological type and lymph node metastasis(P>0.05).Conclusions:1、The expression level of miR-1246 in all cervical cancer tissues was higher than that in normal cervical tissues,and the expression level of miR-1246 in stage Ⅲ and Ⅳ was higher than that in stage Ⅰ and Ⅱ.The expression level of RORA in all cervical cancer tissues was lower than that in normal cervical tissues,and the expression level of RORA in stage Ⅲand Ⅳ was lower than that of stage Ⅰ and Ⅱ.The expression level of NF-κB in all cervical cancer tissues was higher than that in normal cervical tissue,and the expression level of NF-κB in cervical cancer tissues of stage Ⅱ,Ⅲand Ⅳ was higher than that of stage Ⅰ.2、There was a negative correlation between miR-1246 and RORA expression,and a positive correlation between miR-1246 and NF-κB expression.3、The expression levels of miR-1246,RORA and NF-κB were correlated with FIGO stage of cervical cancer,but not with age,tumor size,pathological type,and lymph node metastasis of the patients. |
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