Study On Mycoplasma Pneumoniae Infection And Its Drug Resistance Mutation In Children In Guangyuan District,Sichuan Province

Objective:To investigate mycoplasma pneumoniae(Mycoplasma pneumoniae,MP)infection and MP to macrolide-resistance(23 SrRNA mutant strains)in children from 2016 to 2018 in Guangyuan District,Sichuan Province.Methods:From May 2016 to May 2018,clinical data and sputum or throat swabs of 839 children aged 0-14 years with suspected mycoplasma pneumoniae infection in Guangyuan District,Sichuan Province were were collected.The real-time fluorescence quantitative PCR combined with Taqman fluorescence probe technique were used to detect the infection and macrolide-resistance rate of MP whether there are differences in different seasons,gender and age in Guangyuan District,Sichuan Province.Results:1.MP positive was detected in 294 children with suspected mycoplasma pneumoniae infection,with a positive rate of 35.0%.187 cases of 23 SrRNA mutation were detected in MP positive samples,and the drug resistance rate was 63.6%.2.The positive rate and drug resistance rate were significantly affected by different seasons(P<0.05),among which,the positive rate was 47.7%and the drug resistance rate was 75.2%in winter.3.The positive rate of male children was 35.8%,and the drug resistance rate was 63.5%;The positive rate of female children was 34.1%,and the drug resistance rate was 63.7%.There was no statistical difference between sexes(P>0.05).4.MP positive rate increased with age(P<0.05),while age had no significant effect on drug resistance rate(P>0.05).Conclusion:From 2016 to 2018 in Guangyuan District,Sichuan Province,the infection rate of MP in children was 35%,the macrolide-resistance rate of MP to was 63.6%,the infection rate of MP was related to season and age,the macrolide-resistance rate of MP was related to season.The infection rate and macrolide-resistance rate were higher in winter,and the infection rate increased with age.