Effect Of SCRN1 On Invasion And Migration Of Oral Squamous Cell Carcinoma By Regulating PI3K/AKT Signaling Pathway

Objective:To investigate the expression and biological function of Secerninl(SCRN1)in Oral squamous cell carcinoma(OSCC)and its effect on the biological characteristics of OSCC through PI3K/AKT signaling pathway.Methods:(1)The expression differences of SCRN1 in tumor tissues and adjacent tissues were analyzed by bioinformatics method,and the expression differences of SCRN1 in clinical pathological specimens and adjacent tissues of OSCC were verified by immunohistochemistry(IHC);the expression levels of SCRN1 in normal oral keratinocyte cellline(NOK)and OSCC cell lines(CAL27,SCC25,HSC3,UM1,FaDu)were detected by WB method.(2)Two OSCC cell lines with high expression of SCRN1 were selected to silence SCRN1 gene with lentivirus,and then two OSCC cell lines with low expression of SCRN1 were selected to overexpress SCRN1 gene with lentivirus,and the success of virus transfection was verified by fluorescence microscope,Reverse transcription-polymerase chain reaction(RT-PCR)and WB method;cell proliferation test(CCK8 method),plate colony-forming assay,Transwell invasionassayandscratchassay were used to verified the effects of SCRNlexpressionchange on proliferation ability,colony-forming ability,invasion ability and migration ability and of OSCC cells.(3)The effects of SCRN1 silencing or overexpression on PI3K/AKT signaling pathway and its downstream markers E-cadherin,N-cadherin and Vimentin were detected by WB.Results:(1)Theprotein and mRNA levels of SCRN1 in OSCC tissues were significantly higher than those in adjacent tissues;the expression levels of SCRN1 in OSCC cell lines were significantly higher than those in NOK cellline.(2)The ability of proliferation,invasion,migration and colony-forming of OSCC cell lines with high expression of SCRN1 gene was significantly decreased after silencing SCRN1 gene,while the ability of proliferation,invasion,migration and colony-forming of OSCC cell lines with low expression of SCRN1 gene was significantly enhanced after overexpression of SCRN1 gene.(3)The expression levels of total-PI3K andtotal-AKT remainedunchanged,p-AKT,N-cadherin and Vimentin were significantly decreased and E-cadherin was significantly increased in OSCC cell lines with high expression of SCRN1 gene after silencing SCRN1 gene;the expression levels of total-PI3K andtotal-AKT remainedunchanged,p-AKT,N-cadherin and Vimentin were significantly increased and E-cadherin was significantly decreased in OSCC cell lines with low expression of SCRN1 gene after overexpression of SCRN1 gene.Conclusion:(1)Compared with normal tissues and cells,the expression of SCRN1 increased in OSCC tissues and cell lines,suggesting that the increased expression of SCRN1 may be related to the occurrence and development of OSCC.(2)The change of SCRN1 expression in OSCC cell line can significantly affect its proliferation,colony-forming,invasion and migration ability.(3)The changes of SCRN1 expression can affect the activation of PI3K/AKT signaling pathway,and significantly affect the expression levels of EMT related proteins N-cadherin,Vimentin and E-cadherin,suggesting that the effect of SCRN1 on the biological characteristics of OSCC may be related to the abnormal activation of PI3K/AKT signaling pathway and EMT progression induced by PI3K/AKT signaling pathway.