Study On The Mechanism Of Jiawei Lichong Decoction In Treatment Of Hepatocellular Carcinoma Based On Bioinformatics

Objective To investigate the effect of Jiawei Lichong Decoction on HepG2 cell activity,migration and invasion ability of human liver cancer,and analyze the expression difference between HepG2 cells of liver cancer in Jiawei Lichong Decoction group and HepG2 cell mRNA in control group by high-throughput sequencing technology.Methods MTT method was used to detect the effect of Jiawei Li Chong Decoction on the cell activity of liver cancer HepG2 and to establish the optimal effect concentration of drugs;scratch test to detect the effect of Jiawei Li Chong Decoction on the migration ability of liver cancer HepG2 cells;Transwell test to detect the effect of Jiawei Li Chong Decoction on the invasion ability of liver cancer cells.Using high-throughput sequencing technology to analyze the expression profiles of HepG2 cells and control cells mRNA Gawei Li Chong Decoction group,to screen the mRNA,of differential expression and to analyze the functional enrichment(Gene Ontoloty,)of gene ontology for differential mRNA.GO)and KEGG PATHWAY enrichment analysis to analyze the mRNA that play a key role and their molecular functions,use STRING to construct differential gene protein interaction network,and finally use mi RDB prediction to regulate differential gene mi RNA,and use Cytoscape software to construct regulatory network.RESULTS(1)Jawei li chong decoction can inhibit the activity,migration and invasion of human liver cancer HepG2 cells.(2)A total of 98 differentially expressed mRNA,were screened for 62 upregulated and 36 downregulated genes.(3)The main biological processes involved in the differential genes analysed by GO as follows peptide tyrosine dephosphorylation,regulation of polymer metabolic process,regulation of cell process,g protein-coupled receptor signaling pathway,drug response and other biological processes;the differential genes are mainly located in the cytoplasmic part,the cellular solute part,the nuclear part and other cellular components;The differential genes mainly have cell biological functions such as protein binding,enzyme binding,scaffold protein binding,growth factor binding,transcriptional coactivator activity,protein tyrosine kinase activity,protein tyrosine phosphatase activity,ATP binding,GDP binding and so on.KEGG enrichment analysis.(4)PPI network analysis found that the number of CRELD2、SDF2L1、MANF、DDIT3、NR1H4、MBL2、EGR1、DNAJB9、UGT1A1、ITPR1、CYP1A2、ID1、DNAJB11、SLC17A2、HERPUD1、HSPA5、HYOU1、CYP1A1、AHRR、TMBIM6 nodes is the highest,which is the core part of the whole PPI network,which can be further analyzed as the key genes in this study.(5)mRNA-mi RNA regulatory network construction: DDIT3、DNAJB9、SDF2L1、MANF、DNAJB11、HERPUD1、HSPA5、HYOU1 and other 8 differential genes are highly regulated by the mi RNA network.(6)PCR validation: compared with the control group,the expression of seven key genes,such as DDIT3、DNAJB9、YP1A2、DNAJB11、HERPUD1、HSPA5、HYOU1,was significantly inhibited in the Jiawei Li Chong Decoction treatment group;the expression level of SDF2L1、MANF showed increase.CONCLUSION(1)Jiawei Li Chong Decoction can inhibit the activity,migration and invasion of human hepatoma HepG2 cells.(2)Compared with the control group,the expression of seven cancer-related key genes,such as DDIT3、DNAJB9、YP1A2、DNAJB11、HERPUD1、HSPA5、HYOU1,was significantly inhibited in the Jiawei Li Chong Decoction group;the SDF2L1、MANF expression level was increased.(3)Effects of Jiawei Lichong Decoction on inhibiting HCC cell activity,migration and invasion are related to ER stress sensitive molecules.(4)Jiawei Li Chong Decoction may inhibit the HCC.by inhibiting the activity of CYP1A1、CYP1A2 and then inhibiting the activation of precancerous substances.