STIL Promotes EMT Of Bladder Cancer Under The Control Of YY1

Objective: STIL plays an important role in the process of centrosome replication.In the process of cell mitosis,too much or too little STIL will cause abnormal number of centrosome,which will lead to abnormal cell division.So we speculate that STIL may make important sense in tumorigenesis.A large number of studies have also shown that STIL is highly expressed in a variety of cancer tissues,but its effect on bladder cancer is not clear.We try to verify the correlation,occurrence and development between STIL and bladder cancer.Meanwhile we try to clarify the biological effect of STIL in bladder cancer and possible related pathways.Methods: For this study,we analyzed the correlation between STIL and bladder cancer through bioinformatics.Meanwhile we analyzed the expression of STIL in normal bladder tissue and bladder cancer tissue.Human bladder cancer cell line EJ was cultured in vitro,and STIL specific si RNA was used to transfect cells to inhibit the expression of STIL.Western blot was used to detect apoptosis proteins after inhibiting the expression of STIL.Cell scratch test and Transwell test were used to verify the change of migration and invasion ability after inhibiting the expression of STIL.The expression of EMT related markers was detected by RT-qPCR and Western blot to verify the effect of inhibition of STIL on EMT in bladder cancer cells.In order to further explore the mechanism of STIL,it was found that YY1 was highly expressed in bladder cancer and also positively correlated with the expression of STIL.Western blot was used after inhibiting the expression of STIL to detect the expression of YY1.and the expression of STIL after inhibiting the expression of YY1.Results: The bioinformatics showed that STIL was highly expressed in bladder cancer.After using the si RNA to inhibit the expression of STIL,the apoptosis of cells was inhibited,and the invasion and migration ability of cells were decreased.The expression of EMT related markers detected by RT-q PCR and Western blot showed that the expression of E-cadherin was increased,and the expression of N-cadherin,Vimentin and Zeb-1 was decreased.Compared with normal bladder tissue,YY1 is highly expressed in bladder cancer,which is consistent with the expression of STIL in bladder cancer.The expression level of YY1 in bladder cancer was positively correlated with that of STIL.Either inhibition or overexpression of STIL did not change the expression of YY1 protein.However,the expression of stil protein was decreased after inhibiting of the YY1.The expression of E-cadherin was increased,and the N-cadherin,Vimentin and Veb-1 were decreased after the inhibition of YY1 expression.Conclusion: 1.The STIL protein is highly expressed in bladder cancer.2.The STIL protein can promote the EMT process of bladder cancer.3.YY1 is highly expressed in bladder cancer.STIL may be a downstream target gene of YY1,and can promote the process of EMT in bladder cancer.