| Breast cancer is the highest incidence and mortality cancer among female populations,causing serious harm to patients’ physical and mental health.Triple negative breast cancer(TNBC)is currently the most difficult cancer subtype to treat because TNBC cells lack target receptors for conventional breast cancer drugs.It is necessary to understand the molecular mechanism of these cancer cells in depth and find new targets for drug development.In this subject,the effect and mechanism of forkhead box M1(FOXM1)on breast cancer cells were studied firstly,and then the molecular mechanism of FOXM1 regulated by KDM4B in breast cancer was investigated.This subject has found that FOXM1 is highly expressed in breast cancer cells,and has the highest expression level in TNBC,which plays a role in promoting cancer.Knockdown FOXM1 in MDA-MB-231 cells resulted in a decrease in cell proliferation,migration and colony formation.Meanwhile,the transcription activity of YAP1 protein,which in the downstream of the Hippo signaling pathway,was decreasing and caused cell stemness lower.FOXM1 promoted the expression of hsa-miR-19b-1-5p,which miRNA inhibits the Hippo signaling pathway by targeting the LATS1 gene.Furthermore,the expression of KDM4B was downregulated in MDA-MB-231 cells.It made H3K36me3 markers accumulating,and promoting PHGDH protein expression.PHGDH protein further promoted FOXM1 expression,thereby enhancing the proliferation capacity of MDA-MB-231 cells.In this subject,it was found that FOXM1 could provide new ideas for the treatment of breast cancer by inhibiting the molecular mechanism of Hippo pathway to promote the transcriptional activity of YAP 1 and the molecular mechanism of FOXM1 regulated by KDM4B.In addition,the special miRNA found in this paper,hsa-miR-19b-1-5p,is related to breast cancer,and may be used as a diagnostic biomarker or epigenetic target for breast cancer in the future,which can help breast cancer treatment. |
