| Objective: The main purpose of this project is to verify the difference between the expression of aryl hydrocarbon receptor protein in varicocele and normal male semen and their relative expression in the two groups,and its relationship with clinical data features and relevant parameters of routine semen analysis.The risk factors of infertility in patients with varicocele were also studied.Methods: This study is divided into two parts:(1)verify the expression of aryl hydrocarbon receptor protein in varicocele and normal male semen and the differences between the two groups,and its relationship with clinical data features and relevant parameters of routine analysis of semen;Study on the risk factors of infertility in patients with varicocele.(1)To verify the expression of aryl hydrocarbon receptor protein in varicocele and normal male semen and the differences between the two groups,and its relationship with clinical data characteristics and relevant parameters of routine semen analysis.(1)Collect semen specimens from patients with varicocele and healthy males,use semen analysis and Western Blot to detect semen specimens,obtain semen parameters,verify the expression of Ah R in semen,and determine the relative expression of the two groups;(2)Compare the differences in semen parameters,Ah R expression and clinical data characteristics between patients with varicocele and healthy men,and perform a hierarchical analysis of the relative expression of Ah R in the group of patients with varicocele,and complete the comparison with Correlation analysis between clinical data features and routine semen analysis parameters;(2)Study on the related risk factors of infertility in patients with varicocele(1)The patients with varicocele were divided into two groups according to whether they suffer infertility;(2)Collect the characteristics of clinical data,routine analysis parameters of semen and relative expression of Ah R,and use binary logistic regression analysis to study the risk factors related to infertility in patients with varicocele;Results: ⑴ Verify the expression of Ah R protein in varicocele and normal male semen and the differences between the two groups,and its relationship with clinical data features and relevant parameters of routine semen analysis.(1)According to the inclusion and exclusion criteria,35 healthy adult men were collected in the health examination center as a healthy control group.Among the 90 patients with left varicocele,26 patients with gradeⅠvaricocele(43%)and 28 with gradeⅡ(42%),grade Ⅲ 36(40%),64 fertile patients and 26 infertile patients.Compared with healthy adult men,men with varicocele have lower sperm motility,less percent normal sperm morphology,and lower sperm concentration;(2)Wester blot was used to verify the expression of aryl hydrocarbon receptor protein in semen and determine its relative expression.The results showed that: both the control group and the spermatic vein male had the expression of aryl hydrocarbon receptor protein;Compared to healthy men,men with varicocele had lower Ah R expression(p <0.001);(3)The results of subgroup analysis showed that the relative expression of Ah R in patients with varicocele grade III was significantly lower than that in patients with grade Ⅰ and II varicocele(p <0.001),but there was no significant difference in Ah R expression between patients with grade Ⅰ and(P = 0.16);Compared with the fertile group,the sperm motility of men in the infertile group was lower,the sperm morphology was more normal,and the sperm motility was lower;(4)The results of Pearson correlation analysis showed that the expression of Ah R was significantly positively correlated with sperm concentration and sperm motility in patients with varicocele group.It was significantly negatively correlated with the diameter of spermatic cord and the percentage of abnormal morphology sperm during valsalva maneuver;(2)Study on the related risk factors of infertility in patients with varicocele(1)Binary logistic regression to evaluate the severity of sperm,semen volume,sperm concentration,total vitality,abnormal sperm morphology,and related expression of Ah R,etc.The sensitivity of the model was 69.2%,the specificity was 88.6%,the positive predictive value was 81.8%,and the negative predictive value was 88.2%.The results showed that the risk of infertility in grade III patients was 1.67 times that of patients in grades I and II.For each unit of abnormal sperm morphology,the risk of infertility increased 1.04 times.For each unit increase in sperm concentration,total sperm motility,and aromatic hydrocarbon receptor protein expression,the risk of infertility decreases by 3%,9%,and 11%,respectively;Conclusions: ⑴ Compared with healthy adult men,men with varicocele have lower sperm motility,less normal sperm morphology and lower sperm concentration;⑵ Ah R protein expression was present in both control men and men with varicocele;men with varicocele had lower Ah R expression compared to healthy men;⑶ Compared with the fertile group,the sperm motility of males in the infertile group is lower,the sperm morphology is normal,the sperm motility is lower,and the relative expression of Ah R is significantly lower than that in the fertile group;⑷ Correlation analysis results showed that: Ah R expression in patients with varicocele group was significantly positively correlated with sperm concentration and sperm motility;significantly negatively correlated with the diameter of spermatic veins and the percentage of abnormal morphology sperm during valsalva maneuvers;⑸ The research results of related risk factors show that the risk of infertility of patients with grade III is 1.67 times that of patients with gradesⅠand Ⅱ.For each unit of abnormal sperm morphology,the risk of infertility increases 1.04 times.Sperm concentration,total sperm viability,and each increase in the expression of Ah R protein decreases the risk of infertility by 3%,9%,and 11%,respectively;... |
PDF Full Text Request