Effects Of Micro-/nanostructure Of The Zein Electrospinning Fibers On Cells Under Fluid Environment

Cardiovascular disease is the leading cause of death in non-infectious diseases around the world.Implantation of artificial blood vessels is one of its treatments.Studies have shown that the formation of intact monolayer endothelial cells on the inner surface of the artificial vascular lumen can effectively prevent thrombosis and reduce intimal hyperplasia,and maintain long-term patency after implantation of the artificial blood vessel into the body.Endothelial cells are difficult to resist blood flow impact because of its poor adhesion to the inner surface of artificial blood vessels.Therefore,it is a key problem in artificial blood vessel research to improve its adhesion by regulating the morphology of the inner surface of the stent.In this paper,the zein fiber films with different surface micro-/nanostructures were constructed by electrospinning technology,and its surface micro-morphology,anti-swelling modification effect and regulation of cell morphology were characterized and evaluated.The pre-designed parallel plate flow chamber device was modified to simulate the blood flow environment.The morphology and adhesion of EAhy926 human umbilical vein cell fusion cells on the surface of the fiber films were studied under the shear stress of about 15 dynes/cm~2.The results show that medium and large diameter oriented zein electrospun fiber surfaces can regulate the morphology of endothelial cells(EAhy926)and align the cells;compared with the flat film,the fiber films can improve the adhesion of endothelial cells.When the fiber direction is parallel with the fluid direction,the medium diameter oriented fiber film can significantly improve the ability of resisting shear stress of endothelial cells,and there is a significant difference at each time point after comparison with the small diameter and large diameter oriented fiber films;when the fiber direction is perpendicular to the fluid direction,the medium diameter oriented fiber film can also significantly improve the ability of resisting shear stress of endothelial cells under the short-time shearing stress(1h and 2h).Conclusion:By changing the diameter and arrangement of electrospun fibers,the effects of controlling cell morphology and resisting shear stress can be achieved.