The Function Of ANTXR1 In The Proliferation And Metastasis Of Gastric Cancer And Its Mechanism

Objective Patients with gastric cancer commonly have a poor prognosis,owing to its invasiveness and distant metastasis.Anthrax toxin receptor 1(ANTXR1),a type I transmembrane protein,known as one of receptors that facilitate the entrance of anthrax toxin into cells.Previous studies have confirmed the pivotal role of ANTXR1 in progression and tumorigenesis of diverse cancer types.However,the biological function of ANTXR1 in gastric cancer(GC)is still unknown.The present study aimed to investigate the role of ANTXR1 in GC and illuminate the potential molecular mechanisms.Methods Quantitative real-time polymerase chain reaction(q PCR),western blotting analysis,bioinformatical data mining and immunohistochemistry were employed to examine ANTXR1 expression in GC cell lines and tissues.A series of in vitro and in vivo assays were performed through strategies of loss/gain-of-function and rescue assays to demonstrate the function of ANTXR1 and its possible mechanisms in GC.Results ANTXR1 was upregulated in GC cells and tissues at both m RNA and protein level.High ANTXR1 expression was positively associated with T stage(P=0.001),lymph node metastasis(P=0.002)and clinical stage(P<0.001)in GC patients.Bioinformatics analysis found that ANTXR1 expression was significant upregulated in GC tissue and its overexpression was associated with poor prognosis of GC patients.Further study indicated that ANTXR1 induced proliferation,cell cycle progression,invasion and migration,tumorigenicity and induced suppressed apoptosis in GC.Mechanistic investigation demonstrated that ANTXR1 exerted its promoting effects on GC through activation of the PI3K/AKT/m TOR signaling pathway.Conclusions In conclusion,our findings suggested that ANTXR1 plays a crucial role in the development and progression of GC and may serve as a novel prognostic biomarker and potential therapeutic target for GC.