Study Of Wharton’s Jelly/PCL Electronspun Nanofibrous Scaffold Combined HADSCs For Tissue Engineering Cartilage Construction

Objective: The tissue engineering of cartilage represents a promising approach for cartilage repair,and the process includes cells,tissue microenvironment,and a scaffold.The scaffold is important for supporting,reinforcing,and organizing the regenerating tissue.Currently,Wharton’s jelly(WJ)is considered an ideal scaffold material for cartilage tissue engineering because it has a similar composition,structure,and function as cartilage tissue.In this study,we aimed to fabricate electrospun nanofibers composed of a decellularized WJ matrix reinforced by Polycaprolactone(PCL)and investigate the feasibility of a fibrous WJ/PCL scaffold with h ADSCs for use in cartilage regeneration.Method: WJ was decellularized and then electrospuned with PCL to form a WJ/PCL scaffold.Then,the scaffold was evaluated to determine its microstructures,mechanical properties,hydrophilicity,degradation,immunology,and biocompatibility.Moreover,we evaluated the scaffold’s chondroconductive capabilities with h ADSCs over a period of 4 weeks in vitro.Results: The results indicated that the WJ/PCL scaffold possessed appropriate mechanical properties,excellent hydrophilicity,an increased adherence rate,suitable biodegradation,low immunological reactivity,and good biocompatibility.More importantly,the scaffold showed chondroconductive capabilities with h ADSCs in vitro.Conclusion: The results demonstrated that WJ/PCL is an appropriate scaffold for cartilage tissue engineering.