Using Agent-Based Model To Explore The Effect Of Dexamethasone Endotoxin-induced Inflammatory Respose Of Macrophage

Objective: Perioperative dexamethasone has been found to have various advantages,such as anti-inflammation and inhibiting postoperative nausea and vomiting.However,the exact effects of dexamethasone on the inflammatory response are not clear.This research aims to explore the effects of different doses of dexamethasone on inflammatory response of macrophage inducing by various doses of endotoxin using in silico model.Methods: Developing a simple agent-based model of endotoxin-induced inflammatory response of macrophage on the Net Logo platform.Verifying the effects of different doses of dexamethasone on inflammatory response of macrophage inducing by various doses of endotoxin with clinical and laboratory data reported in the literature.Results: Low-dose dexamethasone can promote macrophages to fight against lowdose endotoxin.However,when macrophages facing high-dose endotoxin,dexamethasone cannot help macrophages to clear endotoxin,which may lead to a continuous inflammatory response,although it can help the cell to transform from M1 type to M2 one temporarily.Furthermore,high-dose dexamethasone may delay the inflammation inducing by endotoxin "second strike",but shows no signs of abating.Conclusion: We have established effective agent-based model of endotoxin-induced inflammatory response of macrophage.The model reveals the potential effect of perioperative dexamethasone dose on postoperative inflammatory response,and is able to provide in silico data for future animal or clinical research.