| Background: Juvenile Recurrent Parotitis(JRP)is a non-obstructive parotitis inflammation common in children.Frequent attacks of JRP affect the normal life of children,but there is no specific treatment and the treatment effect is poor.The main reason that affects the therapeutic effect of JRP is that the etiology and pathogenesis of JRP are unknown,and the current view is that the synergistic action of multiple factors leads to the occurrence of JRP.Objective: Make a retrospective analysis of the etiology of JRP to identify the closely related factors of the pathogenesis of JRP,and probe into the underlying pathogenesis of JRP,so as to provide new ideas and scientific basis for its treatment and prevention.Methods: 1.From 2016 to 2018,131 patients diagnosed with JRP in the department of Oral Surgery of Shanghai Ninth People’s Hospital affiliated to Shanghai Jiaotong University,the school of medicine were included in the study.2.To explore the association between recurrent parotid gland and immune function in children,the laboratory immune function indexes of JRP and normal children were compared.The experimental group consisted of 100 JRP patients diagnosed in the ninth hospital,and the control group consisted of 100 normal children with no history of chronic mumps or mumps.3.To explore the association between children’s recurrent mumps and soft food habits,○1 Clinical research: the masticatory efficiency of JRP and normal children was detected by weighing method.The experimental group was 120 JRP patients diagnosed in the ninth hospital,and the control group was 120 normal children without history of chronic mumps and mumps.○2 Animal research: the mouse animal model of soft diet was constructed,and the effects of soft diet on the function and development of salivary glands in mice were observed from the functional,morphological,histological and molecular levels by feeding food with different hardness but the same nutritional value.Results: 1.According to the analysis of the medical history,the incidence of JRP in the preterm group was higher than that in the full-term group,showing a significant difference.The NK cell number of children with JRP in the early onset group(first onset age <4 years)was significantly reduced,while the Ig E expression level was significantly increased,compared with that in the late onset group(first onset age >4years).The children with soft food habit of JRP had earlier onset age and high recurrence frequency.2.The results of immune function examination showed that the expression levels of Ig G,Ig A,Ig E and CD4 positive T cells in children with JRP were all higher than those of normal children,while the expression levels of CD8 positive T cells were lower than those of normal children.3.Children with JRP prefer soft food diet more than normal children,and their chewing efficiency is also lower than that of children in the normal group.In animal experiments,the size,weight,salivary flow rate and salivary amylase content of the submandibular gland of the experimental group were all lower than those of the control group.Under the ultrastructure,the expression levels of golgi bodies and secretory granules in the submandibular gland of the experimental group were lower than those of the control group.The expression levels of EGF,FGFr2 and Wnt3 a in the submandibular glands of the experimental group were lower than those of the control group.Conclusions: 1.A retrospective analysis of the medical history of JRP shows that the etiology of JRP is significantly correlated with the immune system and the soft food diet.2.Children with JRP have the function of immune deficiency,and cellular immune disorders and hypersensitivity reactions are involved in the pathogenesis of JRP.3.Children with JRP generally have the habit of soft food,and their bad habits lead to the low chewing function of children with JRP.The establishment of soft food animal model showed that the soft food diet could reduce the function and development of salivary glands in mice. |
