| Research purposesCerebral palsy is the most common physical disability in childhood,and its clinical features include movement-related disorders,abnormal posture,cognitive impairment,sensory defects,and many other abnormalities.The causes of cerebral palsy are complex and diverse.Risk factors include premature delivery,perinatal infections(especially chorionitis),intrauterine growth restriction,acidosis or asphyxia before rupture of membranes,multiple pregnancy,etc.,any one or more of the reasons will be Cause brain damage.In recent years,through the transplantation of stem cells and stem cell-related intervention measures(such as human amniotic membrane mesenchymal cell conditioned medium)research,to find more effective treatment methods for the treatment of cerebral palsy-related diseases.research contentCerebral palsy involves a series of complex pathophysiological changes.Humans have never stopped actively exploring the cause,clinical features,pathophysiological changes,clinical diagnosis,treatment,and prevention of cerebral palsy.Brain tissue is mainly related to ischemia and hypoxia.change.Human amniotic mesenchymal stem cells(h AMMSCs)have a wide range of access,convenient acquisition,promotion of paracrine function,no teratogenicity,small immunogenic response,high differentiation potential and multidirectionality,no harm to the collector,The advantages of minimal moral and human relations make amniotic membrane mesenchymal stem cells as a new potential treatment method for the treatment of cerebral palsy.Mesenchymal stem cells can repair damaged or degraded neurons and help brain damage repair and neurological rehabilitation.,Improve spatial learning and memory and other functions,more and more evidence shows that hypoxic-ischemic related symptoms can be actively improved by transplantation of h AMMSCs.The conditioned medium(MSC-CM)obtained in the process of culturing h AMMSCs can have a positive effect by promoting angiogenesis and improving tissue function.The CM secreted by stem cells contains a large amount of anti-apoptosis and a variety of positive growth factors,including liver Cell growth factor(HGF),angiogenin,interleukin series,stromal cell-derived factor 1(SDF-1),insulin-related factors,vascular endothelial factors,etc.For the treatment of cerebral palsy,scholars at home and abroad have conducted in-depth research on the plasticity,regeneration and functional reorganization of the brain,such as rehabilitation training,acupuncture and moxibustion combined with rehabilitation training,electrical acupuncture stimulation of the head movement area combined with rehabilitationtraining,and early enrichment of environmental stimulation And so on the brain development of cerebral palsy and recovery of limb function.In this study,human placental amniotic membrane-derived mesenchymal stem cells(h AMMSCs)and CM were injected into cerebral palsy model mice through the tail vein,and then whether the head electric acupuncture stimulation was used at the same time was used for statistical analysis and analysis of related indicators of neurological function evaluation.The brains of experimental mice were made pathological sections to analyze and compare the neurological rehabilitation effects of cerebral palsy mice.Research resultThe neurological evaluation results of the stem cell treatment group and the CM treatment group were better than those of the PBS control group,and the curative effect of CM treatment and head electric acupuncture stimulation was better than that of the CM treatment group only.Analysis conclusionHuman amniotic membrane mesenchymal stem cells and conditioned medium can improve exercise and spatial memory abilities against cerebral palsy injury,and have a good effect on promoting neurological rehabilitation.The treatment of neurological rehabilitation related to cerebral palsy can be achieved by giving conditioned medium and simultaneously applying head electric acupuncture stimulation. |
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