Determination Of Fluvoxamine In Human Serum By Two-dimensional Liquid Chromatography

BackgroundBlood concentration determination of fluvoxamine is an indispensable scientific data in the process of personalized and rational use of fluvoxamine.The accuracy of the determination results directly affects the administration plan.At present,fluvoxamine is often used in combination with other antipsychotics or other somatic drugs in the treatment of psychiatric patients,which requires a high separation chromatography method to monitor the blood concentration of fluvoxamine.Although liquid chromatography-mass spectrometry（LC-MS）,which is relatively advanced has the characteristics of high sensitivity and good accuracy,the maintenance and analysis cost of the instrument are high,which makes it difficult for primary hospitals to carry out.Conventional one-dimensional chromatography has insufficient separation degree and often requires complex pretreatment,which is difficult to meet the needs of clinical drug monitoring.Therefore,in view of the above problems,this study investigated the clinical monitoring of fluvoxamine plasma concentration in human body by two-dimensional liquid chromatography.ObjectiveA two-dimensional liquid chromatography method with good separation and automatic column switching technology,which has the characteristics of stable,reliable,strong anti-interference ability,low analysis cost,high accuracy,sensitivity and suitable for rapid detection of clinical routine was established for determination of fluvoxamine concentration in human blood,and can provide reference for clinical individualized drug use.MethodsFirstly,a method for the determination of fluvoxamine concentration in human body by two-dimensional liquid chromatography was established:Sample by acetonitrile to protein processing,high-speed centrifugal（14500 rpm）after 8 min,take on the sample 500μL,sample in the first place in the first dimension of cation exchange chromatography column(Aston^TMSCX,3.5×25 mm,5μm)on the concentration and primary separation of target material（the material within the transfer time window）displaced by the automatic switching device in trap column(Aston^TM SRR C₁₈,3.5×10 mm,3μm)in focus,and then the target is cleared into the second dimension of reversed phase chromatographic column(Aston^TM SE C18,4.6×100 mm,5μm),the target was further separated and analyzed on the second dimension chromatography,and finally detected by ultraviolet（UV）detector.The column temperature was 40℃,and the detection wavelength was 253 nm.The results were calculated by external standard method;Secondly,the established method was validated by methodology;Finally,the monitoring of fluvoxamine therapy drugs was applied in clinical practice.ResultsThe method for determination of fluvoxamine concentration in human body by two dimensional liquid chromatographywhich transfer time window was 1.65 to 2.65 min,the linear range of the standard curve was 32.45 to 901.35 ng·m L^-1,the minimum limit of quantitation was 10.54 ng·m L^-1,and the minimum limit of detection was 5.24 ng·m L^-1,RSDs of intra-day and intra-day shall not exceed 2.32%,the recoveries were in the range of 95.7～102.5%,it hasgood stability under various experimental conditions.The monitoring of fluvoxamine therapy drugs has been initially applied in clinical practice,the results show that fluvoxamine can affect the metabolism of combined drugs in human body,and individual drug use is needed clinically.ConclusionsThis experimental analysis method established for the determination of blood concentration of fluvoxamine by two-dimensional liquid chromatography is specific,good accuracy,high stability,and can meet the requirements of in vivo drug analysis,on the basis of fluvoxamine treatment drug monitoring work can guide clinical rational drug use to provide the scientific basis for quantitative,and is suitable for the basic-level hospital.