| Objective:By observing the effect of Liancao Xieli Capsule on the intestinal mucosal barrier of UC model rats,the mechanism of its action on this disease was explored,so as to provide a new scientific basis for the treatment of UC.Methods:After two weeks of feeding,SD rats were randomly divided into blank group,model group,mesalazine group,Liancaoxili capsule low-dose group,Liancaoxili capsule medium-dose group,and Liancaoxili capsule high-dose group.The UC rat model was established by using DSS modeling method.Abdominal aorta blood collection and sampling were performed 7 days later.The general state,body weight and food intake of rats were observed daily,and the disease activity index(DAI)was scored.The length of the colon was recorded,and the gross tissue of the colon was observed under the naked eye and the pathological images of HE staining were observed under the electron microscope.The contents of TNF-α,IL-1β,IL-6,IL-8,IL-17 and INF-γ in serum and intestinal tract of rats were detected by ELISA.The protein expression of ZO-1 and Claudin-1 in intestinal tissues of rats was detected by immunohistochemistry and the m RNA expression of ZO-1 and Claudin-1 was detected by real-time quantitative PCR.Results:(1)Compared with the model group,the body weight of rats in Mesalazine group,LH group,LM group and LL group increased significantly,with statistical significance(P<0.01,P<0.01,P<0.01,P<0.05).The body weight loss induced by DSS was inhibited in Mesalazine group,LH group,LM group and LL group.(2)Compared with the model group,the food intake of rats in the mesalazine group,LM group and LH group was significantly higher than that in the model group,and the difference was statistically significant(P<0.01),indicating that the decrease of food intake in the mesalazine group,LM group and LH group could be inhibited.(3)Compared with blank group,DAI score index of model group was significantly increased(P<0.01).Compared with model group,DAI score index of mesalazine group,Liancaoxaili capsule high-dose group and medium-dose group was significantly decreased,with statistical significance(P<0.01,P<0.01,P<0.05).(4)Compared with model group,mesalazine group,LL group,LM group and LH group could effectively reduce the levels of TNF-α,IL-1 β and IL-8 inflammatory factors in serum of rats(P<0.01).Mesalazine group,LM group and LH group could effectively reduce the levels of IL-6,IL-17 and INF-γ inflammatory factors(P<0.01).(5)Compared with model group,the contents of TNF-α,IL-1β,IL-6,IL-8 and IL-17 in intestinal mucosa of rats in mesalazine group,LM group and LH group were significantly decreased(P<0.01).The levels of colon INF-γ inflammatory factor in LH and mesalazine groups were significantly decreased(P<0.01).(6)Compared with LL group,LM group,LH group and Mesalazine group,the length of colon in model group was significantly shorter(P<0.01).(7)Through microscopic observation,compared with the model group,the structure of each layer of colonic mucosa in the mesalazine group and the Liancaoxieli capsule dosages group was clearer,and the situation of congestion and edema,mucosal ulcer or erosion were significantly improved.(8)DSS induction can reduce the expression of claudin-1,ZO-1m RNA and their corresponding proteins in colon tissue of rats,and the expression level of tight junctin in colon of rats in mesalazine group,medium-dose and high-dose Lianxieli capsule groups was significantly increased(P<0.01).Mesalazine group and Liancaoxili capsule groups could significantly restore the expression level of colon genes in rats(P<0.01).Conclusion:(1)The experimental study showed that Liancao Xili Capsule had a strong anti-inflammatory effect on UC rats made by DSS modeling method.(2)Lian cao xie li capsule can effectively relieve the symptoms of ulcerative colitis such as bleeding and ulcer,reduce the inflammatory reaction of intestinal mucosa tissue,and promote the repair of intestinal mucosa. |
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