Study On The Change Of Serum Hmgb1 Response Under The Intervention Of Different Acupoint Stimulation Therapy

Objective:As a physical stimulus,acupuncture can cause moderate trauma to the acupuncture points.The team’s early animal experiments found that acupuncture,cupping and other acupoint stimulation treatments can cause local muscle fiber rupture,capillary rupture,red blood cell,inflammatory cell infiltration and other micro-damage phenomena in the acupoint tissue.Further studies have found that the trauma caused by acupuncture can cause high expression of the acupoint injury-related molecule HMGB1(High mobility group box-1protein,high mobility group protein 1),and the acupoint HMGB1 participates in the acupuncture effect.However,the results of this study have not been confirmed in humans.In addition,the increase in HMGB1 in the acupoint area has not been confirmed whether the same increase in serum.Therefore,on the basis of affirming the previous research results,this research will carry out further research from the acupoint area to the serum,from the animal to the human body.Observe the changes of serum HMGB1 after acupuncture through human experiments,provide a basis for the study of the principle of acupuncture,provide experimental basis for better application of acupuncture and moxibustion,and promote the application and development of acupuncture and moxibustion.Methods:1.Study on the Response Change of Serum HMGB1 in Mice and Rats Intervened by Different Acupoint Stimulation Therapy:Three different acupuncture points,namely hand acupuncture(twisted acupuncture and non-twisted acupuncture),cupping,and electroacupuncture were applied to normal rats,hypoxic rat models,Alzheimer’s mouse models,and depression rat models.ELISA was used to detect the changes in the protein content of the damage-related molecule HMGB1 in the serum.2.Experimental study on the effect of acupuncture on serum HMGB1 content in healthy people:Carry out research with healthy people as the research object.A total of 46 healthy subjects were enrolled.The experimental group(n=24)used acupuncture as an intervention measure,acupuncture once a day for a total of 7 days;the control group(n=22)did not do manual acupuncture intervention.Before the intervention,2 hours after the end of the 4th day of the intervention,and 2 hours after the end of the 7th day of the intervention,3-5 m L of peripheral blood was drawn from the forearm of the two groups of subjects,and the serum damage-related molecules HMGB1 and chemokine CXCL1 protein were detected by ELISA Changes in content.3.Experimental study on the effect of acupuncture on the serum HMGB1 content in patients with cervical spondylosis:Taking patients with cervical spondylosis as the research object,conduct self-control study before and after.A total of 30 cases were enrolled and treated with acupuncture as a treatment measure,5 times a week,10 times in total.NPQ(Northwick Park Pain Questionnaire)and SF-MPQ(Short-form of Mc-Gill pain questionnaire)are used as curative effect evaluation indicators.Evaluation was performed at the 5th time and the 10 th treatment.Before treatment and 2 hours after the 10 th treatment,3-5 m L of peripheral blood was drawn from the forearm.ELISA was used to detect the changes in serum levels of injury-related molecules HMGB1 and chemokine CXCL1.Results:1.Study on the Response Change of Serum HMGB1 in Mice and Rats Intervened by Different Acupoint Stimulation Therapy:(1)Normal rats: After acupuncture intervention,compared with the blank group,the expression of serum injury-related molecule HMGB1 increased to varying degrees in the twisted needle group and the non-twisted needle group.Among them,the twisted needle group was significantly higher and statistically different(P<0.01),and the untwisted needle group had a rising trend but no statistical difference(P>0.05);compared with the untwisted needle group,the twisted needle group increased and There are statistical differences(P<0.05).After cupping intervention,compared with the blank group,the expression of serum injury-related molecule HMGB1 in the cupping group had a rising trend but there was no statistical difference(P>0.05).(2)Hypoxic rats: Compared with the model group,the expression of serum injury-related molecule HMGB1 in the cupping group had a rising trend,but there was no statistical difference(P>0.05).(3)Alzheimer’s disease mice: Compared with the model group,the electroacupuncture group has a rising trend but no statistical difference(P>0.05).(4)Rats with depression: Compared with the model group,the expression of serum injury-related molecule HMGB1 in the electroacupuncture group had no obvious trend,and there was no statistical difference(P>0.05).2.Experimental study on the effect of acupuncture on serum HMGB1 content in healthy people:There was no statistical difference between the experimental group and the control group in terms of age,height,weight and other baseline data(P>0.05).In the comparison between the two groups,serum HMGB1 at three time points before intervention,on the 4th day of the intervention,and on the 7th day of the intervention There was no statistical difference in the expression of CXCL1 protein(P>0.05).There was no significant difference in the expression of serum HMGB1 and CXCL1 protein between the two groups at three time points(P>0.05).3.Experimental study on the effect of acupuncture on the serum HMGB1 content in patients with cervical spondylosis:(1)Compared with before treatment,the NPQ scale score and SF-MPQ score decreased significantly at the 5th and 10 th treatments,and there was a statistical difference(P<0.01).The 5th treatment and the treatment Compared with the 10 th time,the downward trend was not significant and no statistical difference(P>0.05).Acupuncture is effective in treating cervical spondylosis.(2)Compared with before treatment,the change trend of serum HMGB1 expression and CXCL1 expression in the 10 th treatment was not obvious,and there was no statistical difference(P>0.05).