Nurr1 Improves The Neurological Prognosis After Cerebral Ischemia-reperfusion Injury By Inhibiting PARP-1

Objective: To explore the role and regulation mechanism of Nurr1 in cerebral ischemia-reperfusion injury(I/R).Methods: MCAO/R and OGD/R models were used to construct the model of cerebral ischemia-reperfusion injury;After in vivo injection of adenovirus associated with Nurr1 over-expression and in vitro transfection of Nurr1 plasmid,TTC staining,related neurological function score and CCK-8were used to detect the changes of cerebral infarction,neurological function and neuronal cell activity in rats;The expressions of Nurr1 and PARP-1 were detected by RT-q PCR and Western blot;TTC staining test,brain water content test,foot slip test and m NSS nerve function score test were used to study the effects of Nurr1 over-expression treatment,PJ-34 treatment and combined treatment on brain tissue injury.Results: After cerebral ischemia reperfusion injury,the expressions of Nurr1 and Nurr1 m RNA were down-regulated,while the expressions of PARP-1 and PARP-1 m RNA were up-regulated.After Nurr1 over-expression,the brain tissue injury and neuronal injury after cerebral ischemia reperfusion injury were significantly alleviated and the expression of PARP-1 was significantly inhibited.Nurr1 over-expression combined with PJ-34 therapy significantly improved the prognosis of the nervous system after cerebral ischemia reperfusion injury.Conclusion: Nurr1 plays an important role in brain repair after I/R injury,which may be achieved by inhibiting PARP-1 expression,thereby reducing cerebral infarction size,promoting neurological recovery and improving neuronal cell activity.Inhibition of PARP-1 expression by regulating the interaction between Nurr1 and PARP-1 may be an effective treatment to reduce neuronal damage and death after cerebral ischemia reperfusion injury.