| Purpose: This topic is mainly to explore the correlation between the expression of peripheral blood mi R-218-5p and the expression level of High-mobility group box-1(HMGB1)in children with Hen(?)ch-Sch(?)nlein purpura(HSP)and Hen(?)ch-Sch(?)nlein purpura(HSPN).And compare with clinical laboratory indicators to analyze the correlation.Explore the biological role of mi R-218-5p and HMGB1 in the pathogenesis of children with HSP and HSPN.Method: From November 2019 to August 2020,30 children with HSP and 30 children with HSPN who were hospitalized in the Pediatrics Department of the People’s Hospital of Inner Mongolia Autonomous Region were selected as the experimental group,and 30 children with healthy physical examination in the same period in our hospital were selected as the control group.Real-time quantitative Polymerase Chain Reaction(RT-PCR)technology detects the expression of mi R-218-5p in peripheral blood.Double antibody one-step sandwich enzyme-linked immunosorbent assay was used to detect the expression of HMGB1 in peripheral blood.To analyze the clinical significance of the expression levels of mi R-218-5p and HMGB1 in children in the HSP group,HSPN group,and healthy control group.Pearson correlation analysis of the expression levels of mi R-218-5p and HMGB1.At the same time,the differences in the expression of WBC,ANC,ALC,RBC,PLT,UREA,CRE,UA between the HSP group and the HSPN group were analyzed.Perform multi-factor correlation analysis on the selected meaningful indicators.Finally,the ROC curve is used to determine the index diagnosis cut-off point and evaluate the index.Results:(1)The expression of mi R-218-5p in the HSP group was lower than that in the control group,and the difference was statistically significant(P<0.05).The expression of mi R-218-5p in the HSPN group was lower than that in the HSP group,and the difference was statistically significant(P<0.05);(2)The expression of HMGB1 in the HSP group was higher than that of the control group,and the difference was statistically significant(P<0.05).The expression of HMGB1 in the HSPN group was higher than that in the HSP group,and the difference was statistically significant(P<0.05);(3)Mi R-218-5p,HMGB1,WBC,ANC,ALC,RBC,PLT,UREA,CRE,UA and other indicators compared with HSP correlation analysis of children with kidney damage.Mi R-218-5p,HMGB1,WBC,ANC and the occurrence of kidney damage are meaningful.However,ALC,RBC,PLT,UREA,CRE,UA have no significance in the occurrence of kidney injury;(4)Binary Logistic regression analysis was performed on the variables with statistical significance in the occurrence of kidney injury.Mi R-218-5p is an independent protective factor for kidney damage in children with HSP.HMGB1 is an independent risk factor;(5)ROC curve is drawn with mi R-218-5p and HMGB1 expression as variables.According to the principle of maximum Youden index,the best cut-off value of mi R-218-5p for diagnosing kidney injury in the development of HSP was determined to be 0.515,the sensitivity was 100%,and the specificity was76.7%.The best cut-off value of HMGB1 for diagnosing kidney injury is 3348.2pg/ml,the sensitivity is 86.7%,and the specificity is 90%.Conclusion: The expression of mi R-218-5p in peripheral blood of children with HSP decreased,and the expression of HSPN group decreased more significantly.The expression of HMGB1 in peripheral blood of children with HSP increased,and the expression of HSPN group increased more obviously.There is a significant negative correlation between mi R-218-5p and HMGB1.Mi R-218-5p may participate in the pathogenesis of HSP and HSPN through targeted regulation of the expression of HMGB1.Mi R-218-5p is an independent protective factor for kidney damage in children with HSP.HMGB1 is an independent risk factor for kidney damage in children with HSP.Therefore,it is speculated that mi R-218-5p and HMGB1 may be indicators for predicting the severity of kidney damage in children with HSP.Provide new biological markers for clinical diagnosis and treatment of HSPN. |
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