| Objective: Chlamydia trachomatis(Ct)is a long-term and widespread pathogen in human body,which often leads to genitourinary tract diseases and eye diseases.There are 19 serotypes of Ct.Serotype D-K is the main pathogen causing nongonococcal urethritis.Epidemiological survey in China shows that infection accounts for about 60% of nongonococcal urethritis.This paper collects the detailed information and information of urogenital tract examiners in the 81 st group military hospital(formerly 251 Hospital of PLA),analyzes the infection of Chlamydia trachomatis(Ct),and classifies the Ct.The classification of Ct helps to determine whether it is a recurrence or reinfection of Ct,and provides the basis for the prevention and treatment of Ct infection;And through the analysis of the epidemiological characteristics and epidemic factors of urogenital tract Ct infection,to provide reference for the local development of effective prevention and control measures.Methods:1 Using cluster sampling method,Zhangjiakou area was selected as the research site,and the outpatient department of Obstetrics and Gynecology of the 81 st group Military Hospital of the Chinese people’s Liberation Army from October 2017 to October 2019 was selected as the research object.A unified patient information questionnaire was designed for basic data collection.At the same time,the medical records were consulted,and the detailed clinical data were obtained as much as possible.1.1 Inclusion criteria1)Non pregnant women;2)The presence of abnormal vaginal discharge and / or urethral irritation;3)No antibiotics and vaginal lavage were used two weeks before admission.1.2 Exclusion criteria1)Pregnant women2)Those who had taken sex hormone in the last 6 months and antibiotics in the last 1 month.2 The cervical infection was detected by RT-PCR.3 Nested PCR was used to amplify the Ct positive samples.The PCR products were sequenced and genotyped.The clinical features and differences of different genotypes were analyzed.4 The vaginal swabs and urine samples of Ct positive patients were further detected by PCR-fluorescent probe method to compare the difference of positive rate from these three types of specimens.5 The spouses of Ct positive patients were tested for Ct at the same time,and the results were analyzed.6 The age,occupation,living area,contraceptive methods and other constituent ratio were statistically analyzed.SPSS 13.0 software was used for statistical analysis of the data.The continuous variables were described by means and standard deviation,and the comparison between the two groups was made by t-test;the counting data was expressed by percentage,and the difference of rates was analyzed by chi square test.P < 0.05 was considered to be statistically significant.Results:1.A total of 1353 cervical swabs were collected.A total of 44 positive samples were obtained by fluorescence PCR,and the positive rate was about3.25%.2.Forty four positive samples were genotyped and 6 genotypes were detected.Among them,15 strains were E type(34.09%),12 strains were J type(27.27%),8 strains were D type(18.18%)and 7 strains were F type(15.91%),Which are the dominant epidemic type.the same as the previous domestic reports.Through the investigation,it was found that there was no correlation between the clinical symptoms and signs of different types of patients and omp1 type.3.There was no significant difference in the results of cervical swab,vaginal secretion and urine Ct in the same patient.4.A positive rate of 65.7% was tested on spouses of positive patients,which indicated that both husband and wife have a high rate of Ct infection at the same time.5.The results showed that female genitourinary tract Ct infection was related to age,occupation and other factors.The infection rate of women under 40 years old was 3.83%.The infection rate of service and workers was higher,with the infection rate of 5.17%.However,the difference between urban and rural areas had little effect on the situation of Ct infection.The infection rate of condom users(1.09%)was significantly lower than that of intrauterine device(IUD),oral contraceptive and non contraceptive(5.79%).Conclusions:1.Among the female outpatients selected in this study,the detection rate of urogenital tract Ct infection was 3.25%,and the detection rate was higher.2.Clinical symptoms and signs were not associated with Ct type.3.Ct routine physical examination,urine or vaginal swab can be collected.4.Both husband and wife should carry out Ct detection and treatment at the same time.5.In Zhangjiakou area,female in the 31-40 age group;service and workers are the high incidence of Ct infection;urban and rural differences are not significant,the use of condoms can effectively prevent Ct genitourinary tract infection. |
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