Analysis Of Whole Transcriptome Changes After Neomycin Injury In Neonatal Mouse Utricle

Objective: The research on hair cell protection and regeneration is of great significance for the prevention and treatment of deafness,vertigo and other diseases.It is an important content of the research on hearing and vestibular protection to find the key gene combination that can promote the inner ear hair cells to resist damage and promote regeneration.With the rapid development of high-throughput sequencing technology and bioinformatics technology,the research on transcriptomics and proteomics of disease models is more in-depth.A large number of studies have reported the expression changes of related coding RNA after cochlear hair cell injury,but there are few reports on the differential expression of non coding RNA.The purpose of this study is to investigate the changes of full transcriptome expression at different time points after vestibular hair cell injury,as well as the signaling pathways and biological processes related to different genes,so as to provide new methods and ideas for hair cell regeneration and protection and vestibular function remodeling.Methods: In order to study the effect of neomycin on the expression of full transcriptome,RNA SEQ was used to detect the expression of full transcriptome.RESULTS: The results showed that 962 lnc RNA and 352 lnc RNA genes were up-regulated and down regulated immediately after neomycin injury;21 and 28 circ RNA genes were up-regulated and down regulated;The intersection of 141 lnc RNA genes up-regulated immediately and 163 lnc RNA genes up-regulated 3 days after neomycin injury was 14;The intersection of 232 lnc RNA genes down regulated immediately and 295 lnc RNA genes down regulated 3 days after neomycin injury was28.Compared with the blank group,the lnc RNA differentially expressed in cancer small molecular RNA,AGE-RAGE and other signaling pathways in diabetic complications were significantly enriched in the immediate neomycin injury group.Significant differentially expressed genes were obtained by predictive screening:Col4a2,Nfatc1,Mir155,Mir29 c,Mir100.Compared with the blank group,the expression of circ RNA in c AMP signaling pathway,cell senescence and other signaling pathways was significantly enriched in the neomycin injury immediate group.Through prediction and screening,the significantly differentially expressed genes were obtained: Vav3,Ppp1 cb,Lin54,Cacna1 d.Conclusion: In this study,transcriptome sequencing was used to analyze the full transcriptome expression profile of the sensory epithelium of the neonatal mouse utricle after injury.Some common biological processes and signal pathways related to the disease were found,some of which may be the target genes and signal pathways affecting hair cell injury and regeneration.The results need further functional and mechanism verification.This study provides some reference information for the follow-up study of hair cell protection and regeneration.