The Effects Of Meprin α On Collagen Synthesis In Lung Fibroblasts Induced By TGF-β1

Objective To explore the effects of Meprin α on collagen synthesis and myofibroblast differentiation in rat lung fibroblasts induced by TGF-β1.Methods The rat lung primary fibroblasts were cultured and divided into 4 groups,including control group,TGF-β1 group,TGF-β1+recombinant Meprin α group,and TGF-β1+actinonin group.Cell proliferation was measured by CCK-8 assay.Cell migration was measured by cell scratch wound test.The expression of α-smooth muscle actin(α-SMA)was observed by immunofluorescence(IF)staining.The levels of Meprin α,α-SMA,and collagen type I(col I)were also measured by western blot.Results 1 Compared with control group,treatment with TGF-β1 incereased cell proliferation(P<0.05).Treatment with meprin α could reduced the increasing cell proliferation in lung fibroblasts induced by TGF-β1(P<0.05).There was no significance between TGF-β1 and TGF-β1+actinonin groups(P<0.05).2 TGF-β1 promoted cell migration in rat lung fibroblasts(P<0.05).Pre-treatment with recombinant meprin αinhibited cell migration in fibroblasts induced by TGF-β1(P<0.05).Furthermore,actinonin increased cell miagration in TGF-β1 treated lung fibroblasts(P<0.05).3 IF staining show a positive expression of α-SMA with red fluorescence.There was no positice expression ofα-SMA in control group.Positive expression of α-SMA could be observed in TGF-β1treated lung fibroblasts.Prtreatment with meprin α decreased the positive of α-SMA and actinonin have the opposite effect.4 The levels of α-SMA and col I were increased in TGF-β1-treated lung fibroblasts,as well as down-regulation of Meprin α(P<0.05).5Pretreatment with Meprin α attenuated increasing levels of col I and α-SMA in TGF-β1-treated lung fibroblasts(P<0.05).The inhibitor of Meprin α,actinonin,promoted the levels of col I and α-SMA in lung fibroblasts induced by TGF-β1(P<0.05).Conclusion Meprin α inhibits the collagen synthesis and myofibroblast differentiation in lung fibroblasts induced by TGF-β1.Figure 6;Table 5;Reference 166...