Exploring The Molecular Mechanism Of Curcuma Phaeocaulis Valeton Anti-stress Response Based On Network Pharmacology

With the continuous changes in the environment,people are exposed to more and more stressors both work and daily life,which has caused harm to human health in recent years.How to prevent and treat stress-induced injury has become a research hotspot already.As the main therapeutic drug of Chinese medicine,traditional Chinese medicine has formed a set of treatment system after thousands of years of development.However,the chemical composition of traditional Chinese medicine is too complex,which seriously hinders the in-depth development of traditional Chinese medicine.The emergence of network pharmacology has made it easier to deeply study the mechanisms of different components of traditional Chinese medicine,which markedly reduce the workload of new drug research and development.As an excellent model organism,C.elegans has been widely used in drug screening.Therefore,network pharmacology was used to screen the target traditional Chinese medicine,and its active ingredients were predicted in the early stage of this study.Then,the C.elegans was used as an in vivo animal model to explore the molecular mechanism of the target traditional Chinese medicine.Firstly,by consulting the literature and screening the online database,Curcuma phaeocaulis Valeton was selected as the target traditional Chinese medicine.Through the network pharmacological analysis of Curcuma phaeocaulis Valeton,it was predicted that Curcuma phaeocaulis Valeton had the potential of anti-stress.The total curcumin compounds in Curcuma phaeocaulis Valeton was predicted to relate to such potential by the topological parameter analysis.Afterwards,different concentrations of Curcuma phaeocaulis Valeton water extract were administered to C.elegans.Through the UV stress experiment,it was found that the 10 mg/m L Curcuma phaeocaulis Valeton water extract had a more stable anti-ultraviolet stress ability than another drug group,which could prolong the average lifespan of C.elegans by 12.99% compared with the control group.Therefore,the concentration of 10 mg/m L was selected in the follow-up experiments.In addition to UV stress,the study found that Curcuma phaeocaulis Valeton water extract can also improve the C.elegans’ s resistance to heat stress,oxidative stress and Pseudomonas aeruginosa infection.Curcuma phaeocaulis Valeton water extract can significantly reduce the Reactive Oxygen Species(ROS)content,and increase the activity and expression of superoxide dismutase SOD-3 in C.elegans.Besides,Curcuma phaeocaulis Valeton water extract can also promote the nuclear translocation of DAF-16,but can not affect the anti-stress ability of insulin/IGF-1-like signaling pathway mutant C.elegans strains CF1588 and CF1038.In addition,q PCR results showed that the increasing expression levels of related gene,e.g.daf-16,hsp-16.49,hsp-12.6 and sod-3,indicating that the water extract of Curcuma phaeocaulis Valeton enhanced the anti-stress ability of C.elegans and was related to the insulin/IGF-1-like signaling pathway.By analyss the effective components in Curcuma phaeocaulis Valeton the total curcumin compounds were considered to be the effective components of Curcuma phaeocaulis Valeton that exerted the antistress function,of which curcumin played a major role.In conclusion,this study found that Curcuma phaeocaulis Valeton can effectively improve the anti-stress ability of model organism Caenorhabditis elegans,and it is related to the insulin-like signaling pathway,These results correspond with the prediction results of network pharmacology.Overall,this research aims to provide ideas for the multi-directional development of traditional Chinese medicine Curcuma phaeocaulis Valeton,which possesses the value of theoretical research and practical application.