Electrochemical Sensors Investigate The Effects Of Integrin αvβ3 Antibodies On Breast Cancer Cell Adhesion And Migration

Breast cancer is the most common malignancy in women worldwide,and metastasis of the primary lesion is the reason for the high mortality rate of breast cancer patients.Cancer metastasis is closely related to the migration ability of cancer cells,and cell adhesion is a precursor to cell migration,so it is of great scientific significance to study the adhesion and migration behavior of cells.Integrin αvβ3 is highly expressed in human breast cancer tumor cells and plays an important role in regulating tumor growth,angiogenesis,local invasion and migration capacity,so the effect of integrin αvβ3 on the adhesion and migration behavior of breast cancer cells was studied by adding the corresponding integrin αvβ3 antibody.In this thesis,a cell adhesion sensor was constructed in vitro to monitor the effect of αvβ3 antibody on the adhesion behavior of MDA-MB-231 cells alone,and then a co-culture model of endothelial cells and breast cancer cells was constructed to simulate the tumor microenvironment in humans,and the effect of αvβ3 antibody on co-culture cell behavior was monitored in real time,and then a cell migration sensor was constructed on the basis of co-culture to study the changes in the adhesion and migration behavior of co-cultured breast cancer cells.This thesis consists mainly of the following parts:First,Construct an electrochemical adhesion sensor to detect the effect of integrinαvβ3 antibody on the adhesion of individually cultured breast cancer cells.Design the ITO chip,use it as a substrate,add a certain amount of cells,combine different concentrations of αvβ3 antibody,and build an electrochemical sensor for detecting dynamic changes in cell adhesion.The monitoring results show that the sensor can well show the adhesion changes of cells under different culture conditions（αvβ3 antibody concentration change）,where the impedance parameter results are consistent with the microscopic characterization results.Thus,this electrochemical biosensor can be used for cell adhesion studies and the optimal αvβ3 antibody concentration is derived by comparing adhesion time to cell coverage.Second,establish a co-cultivation platform in vitro.Simulating the tumor microenvironment in vivo,the transwell chamber co-culture model of breast cancer cells and endothelial cells was constructed,and the effects of co-culture before and after coculture and the addition of integrin αvβ3 antibodies were detected by EIS real-time impedance change,and the impedance value parameters were consistent with the experimental results of CCK8.Third,build an EIS platform for monitoring changes in cell migration capacity.Designing ITO glass chips that can be used for cell scratching operations,the sensors are built to respond to the healing process of breast cancer cells.The analysis of the impedance data shows that the sensor can monitor the change of cell migration behavior under different conditions（co-culture,αvβ3 antibody）,indicating that the constructed electrochemical cell sensor can accurately monitor the change of cell adhesion and migration behavior.