| Objective To explore the effect of bunaofushenfang on bilateral lat eral ventricle injection of Aβ1-42 effects of oxidative stress in AD model rats and its possible mechanism.Methods Fifty male SD rats were randomly divi ded into five groups: Normal group（control）,Sham operation group（sham）,Model group（AD）,Bunaofushenfang group（AD+bnfsf）and Donepezil grou p（AD+dnpq）.The AD model was established by bilateral lateral ventricle in jection of Aβ1-42,the Sham operation group was injected with sterile normal s aline in the same way.7 days after modeling,sham group and AD group we re given double distilled water by gavage,and the AD+bnfsf group and AD+dnpq group were treated with the corresponding drug liquid gavage.The nor mal group was not gavaged,and the gavage continued for 4 weeks.The learni ng and memory ability of the rats in each group was observed by Morris wat er maze;the changes of neuronal cells and Nissl bodies in CA1 area of hipp ocampus of each group was observed by HE and Nissl staining;the contents of H2O2,SOD,GSH-Px and MDA in the serum and hippocampus of the rats in each group were detected by kits;the protein expressions of Nrf2,Keap1 and HO-1 in the hippocampus of rats in each group were detected by Weste rn Blot.Results 1 The results of Morris water maze showed that compared with control and sham groups,the time of looking for the platform in AD gr oup was significantly longer（P<0.01）,the swimming track was more disorder ed,the time of reaching the platform for the first time was longer（P<0.05）,and the number of crossing the platform was significantly reduced（P<0.01）;Compared with AD group,the latency of rats in the treatment group was sho rtened,the time of looking for the platform was shortened（P<0.05）,the tend ency of looking for the platform during swimming,the time of first arriving at the platform was shortened,and the number of crossing the platform was i ncreased（P<0.05）.2.The results of HE staining showed that compared with control group and sham group,the neurons in hippocampal CA1 area in AD group were stained unevenly,arranged disorderly,the number of neurons decr eased significantly,and the neuronal structure was destroyed;After administrat ion,neurons in CA1 area of hippocampus in AD+bnfsf group were orderly ar ranged,the cell spacing increased,the number of neurons increased and the st ructure of neurons was incomplete;In AD+dnpq group,neurons are arranged orderly and slightly loose,the number of neurons is more than that in AD gr oup,and the structure of neurons is basically complete.3.Nissl staining resul ts showed that compared with control group and sham group,the staining col or of neurons in CA1 area in AD group was different,arranged disorderly,a nd the number of Nissl bodies decreased;The neurons in CA1 area in each t reatment group were stained deeply,the cells were arranged neatly,and Nissl bodies were abundant.4.The results of oxidative stress index showed that c ompared with control group and sham group,the contents of H2O2 and MDA in serum and hippocampus increased,and the contents of SOD and GSH-Px decreased in AD group（P<0.05）;Compared with AD group,the contents of H2O2 and MDA in serum and hippocampus decreased,while SOD and GSHPx increased（P<0.05）.5.WB results showed that compared with control grou p and sham group,the expression of Nrf2 and HO-1 protein in hippocampus of AD group decreased significantly,and the expression of Keap1 protein incr eased（P<0.05）;Compared with AD group,the expression of Nrf2 and HO-1in hippocampus increased and the expression of Keap1 protein decreased in e ach treatment group（P<0.05）.Conclusion Bunaofushenfang can improve the l earning and memory ability of AD rats,and has a certain protective effect on the hippocampal neurons of AD rats.The mechanism may be through regula ting the Nrf2 signaling pathway and inhibiting oxidative stress. |
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