Effects Of Salt Stress On The Morphological Characteristics Of Arbuscular Mycorrhiza And Rhizosphere Soil Enzyme Activities In P

The problem of soil salinization in the world currently is a common phenomenon,is also a very difficult problem,seriously restricting the development of agriculture and forestry,and the northeast plain of China there are a lot of saline area.Arbuscular mycorrhizal fungi(AMF)can interact with more than 90%of the host plants on the earth to promote the growth of plants and improve the stress resistance of plants.Fungi.According to previous studies,Elaeagnus angustifolia is a species of salt tolerant plants,AMF and symbiotic against salt stress after angustifolia has a good effect.This paper selects the AMF glomus intraradices(GI),and after the determination of the growth response of Elaeagnus angustifolia L.symbiotic effect in 4 different salt concentrations and forms,to reveal the effects of mycorrhizal seedlings for salt stress tolerance of Elaeagnus angustifolia L.The main results are as follows:1.The infection rate of Elaeagnus angustifolia L.was found in salt and salt angustifolia after infection rate is basically the same,but the infection hyphae and vesicles were observed after the salt quantity decreased;spore density decreased with the increase of salt concentration,and spore morphology by salt stress damage,the greater the concentration the more damage serious;the amount of mycelium is reduced with the increase of salt concentration.2.Increase the concentration of salt resulted in Elaeagnus angustifolia L.plant height,basal diameter,root length and root area have inhibition effect,the higher concentration,the degree of inhibition is more obvious;at the same time of salt stress on the activity of four enzymes related to invertase,urease,phosphatase and catalase also inhibit the enzyme activity,negative associated with salt concentration;the total glomalin was also inhibited by salt stress,and negatively correlated with salt concentration.But the AMF and the mycorrhizal symbiosis of Elaeagnus angustifolia L.,compared with non mycorrhizal plants,significantly increased the content of Elaeagnus angustifolia L.in the growth index,enzyme activity and total glomalin etc..3.Through comprehensive analysis on determination of Na~+content in the roots of Elaeagnus angustifolia and Na~+content in rhizosphere soil,AMF can prevent Na~+to the transport of Elaeagnus angustifolia root,AMF inhibited the absorption of Elaeagnus angustifolia root of Na~+to a certain extent,reduce the degree of injury by Na~+angustifolia.4.Through the observation of Elaeagnus angustifolia L.root cell ultrastructure,effect of salt stress on Elaeagnus angustifolia L.root cells exist ion toxicity and osmotic stress,but the number of organs and cells of Elaeagnus angustifolia L.root morphology of mycorrhizal fungi significantly than non mycorrhizal ones,indicating that AMF can mitigate the symbiosis and Elaeagnus angustifolia L.and salt stress degree,help angustifolia better root growth.The effect of mycorrhizal seedlings is very significant,AMF and Elaeagnus angustifolia L.symbiosis can promote plant growth and improve soil environment,enhance the salt tolerance of plants,to provide technical support for the ecological management of saline area,has important practical significance.