Experimental Study On The Effects Of Mito K_ATP Channel Regulation On The Proliferation And Osteogenic Differentiation Of Murine Bone Marrow Mesenchymal Stem Cells (MSCs)

Objective: OBJECTIVE: To investigate the feasibility of murine mesenchymal stem cells(BMSCs)in osteoblasts and to investigate whether the changes of Mito KATP channels affect the activity of bone marrow mesenchymal stem cells(MSCs)and their differentiation into osteoblasts.Methods: Approximately 100 grams of mice were sacrificed.After the death,the bilateral bone marrow rinse solution was rapidly separated and cultured in vitro.The morphology and growth and proliferation of the cells were observed under inverted phase contrast microscope.(Group A),group B(0.16μmol / L diazoxide),group C(0.8μmol / L diazoxide),group D diazoxide 4μmol /L(group A))Were observed and tested at different concentrations of Mito KATP channel activator diazoxide.The cells were also grouped with different concentrations of Mito KATP channel activator diazoxide for 2 weeks with alkaline phosphate Enzyme,alizarin red and nuclear staining were used to detect the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells(MSCs).Results: The results of primary cell culture showed that the ability of cell purity,viability,proliferation and differentiation were different in different sizes.The mesenchymal stem cells(MSCs),which have been constructed,have higher homogeneity and purity,but the ability of proliferation and differentiation is different.There was no significant difference in the activity of bone marrow mesenchymal stem cells(MSCs)between different concentrations of Mito KATP channel activator diazoxide(P> 0.05).(MS)proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells(BMSCs)by alkaline phosphatase,alizarin red and nuclear staining.Mito KATP channel activator diazoxide solution at a certain concentration 0.16μmol / L diazoxide,0.8μmol / L diazoxide,diazoxide 4μmol / L 8 days in the experimental study does not affect the rat bone marrow mesenchymal stem cells But the three concentrations were significantly lower in the control group than in the control group 14 days after the intervention.In the induction of bone marrow mesenchymal stem cell differentiation,0.8 μmol / L diazoxide and diazoxide 4 μmol / L could significantly induce osteogenic differentiation of mesenchymal stem cells after 14 daysConclusion: Mito KATP channel activator diazoxide does not affect the activity of bone marrow mesenchymal stem cells(MSCs),and a certain concentration(greater than 0.8μmol / L diazoxide)can enhance bone marrow mesenchymal stem cells to osteogenic differentiation...