Paper-based Chemiluminescence Studies Of Bimetallic Nanomaterials/metal-organic Gels And Their Applications

As chemiluminescence(CL)has advantages of simple instrument and high sensitivity,it has benn widely used in the field of clinical diagnosis,environmental monitoring and food safety.Luminol system is one of the common used CL system and horseradish peroxidase(HRP)has good catalytic activity in this system.Due to the natural enzymes has the disadvantages of instability and vulnerability to environment,artificial enzymes which have the properties of oxidase/peroxidase need to be developed.Co-Fe nanocubes and metal-organic gels with the property of mimic enzymes were synthesized.We applyed the materials in CL and colorimetric detection.It realized that the sensitive determination of caffeic,dopamine and glutathione.The paper is mainly divided into two parts.The first part is the review,which mianly introduces the the application of CL,and the paper-based CL device in recent five years.The second part is research report including three works.The research and results are as follows:1.Paper-based chemiluminescence device with Co-Fe nanocubes(Co-Fe PBA NCs)for sensitive detection of caffeic acidIn this work,a new chemiluminesce(CL)system of Co-Fe nanocubes(Co-Fe PBA NCs)which can catalyze luminol to produce strong CL in the absence of H2O2 was established,Co-Fe nanocubes has the property of oxidase-like activity and it can catalyze the generation of reactive oxygen in the dissolved oxygen system.Since the caffeic acid of phenolic compounds can remove reactive oxygen species in the system,a sensitive detection method for caffeic acid(CA)on the paper-based chip was developed.Under the optimal conditions,the method showed good linear response to caffeic acid in the range of 10 ng/mL-800 ng/mL with the limit of 3 ng/mL.The proposed method had been used for the determination of caffeic acid in tea samples.2.Copper-based metal-organic xerogels(Cu-MOXs)on paper for the chemiluminescence detection of dopamineIn this work,copper(?)based xerogels(Cu-MOXs)which is composed of copper as the central ion and 2,2'-bipyridine-6,6'-dicarboxylic acid as the ligand was quickly synthesized under a mild facile strategy.Cu-MOXs exhibited superior catalytic performance for the luminol-H2O2 CL system.The possilble mechanism was studied by CL spectra,UV-Vis absorption and electron paramagnetic esonance(ESR).Since dopamine can inhibit the reaction of this system,a sensitive paper-based CL device for the detection of dopamine was established.Under the optimal experimental conditions,the linear range of this method was 40 nM-200 nM with detection limit of 0.01 ?M.The proposed method had been used for the determination of dopamine in urine.3.Fe(?)bipyridyl or phenanthroline complexes with oxidase-like activity for sensitive colorimetric detection of glutathioneIn this work,three kinds of Fe(?)bipyridyl or phenanthroline(Fe(?)-L3)complexes which can catalyze 3,3',5,5'-tetramethylbenzidine(TMB)to induce chromogenic change in the absence of H2O2 was reported.Fe(?)-L3 complexes can directly make TMB change color with a shorter incubation time.With high oxidase-like activity of Fe(?)-L3 complexes,superoxide anion radical(O2·-)was formed in the solution.Then the intermediates radical involving oxo-iron species were produced and oxidize TMB to its oxidation products(oxTMB).As glutathione(GSH)can inhibit this oxidation reaction of Fe(?)L3 complexes-TMB system,a rapidly colorimetric method was established for the discriminative detection of glutathione(GSH)with the detection limit of 0.1 ?M.We discovered that Fe(?)-L3 complexes can directly catalyze TMB to oxTMB without H2O2.The fast and simple colorimetric method may open a new avenue for the application of point-of-care diagnosis(POCT)field with TMB chromogenic system.