Research On The Chiral Recognition Performance Of Noble Metal Nanoparticles With Different Morphologies

Chirality is a common phenomenon in nature,and chirality recognition is of great significance in life activities.The colorimetric method is fast,simple,does not require large instruments,and is popular in chiral recognition.Nanoparticles with different morphologies show different degrees of difference in optical properties and catalytic properties.In this thesis,Au rhombohedral dodecahedral and Silver nanoprisms were synthesized,and the chiral recognition performance of these two nanoparticles with different morphologies was studied,and then the chiral recognition new method using these two nanoparticles as colorimetric probes was established.1.Research on the chiral recognition performance of Au rhombohedral dodecahedral nanoparticlesAu rhombohedral dodecahedral nanoparticles(AuRD)are gold nanoparticles with a high Miller index surface.In this paper,a traditional seed method was used to synthesize cetyltrimethylammonium chloride(CTAC)coated AuRD,and its structure was characterized by ultraviolet-visible spectroscopy and transmission electron microscopy.Using Histidine(His)as a model chiral molecule,the chiral recognition performance of AuRD was studied.The experimental results show that D-His can change the color of AuRD solution from pink to blue within 2 minutes,while L-His will not change the color of AuRD solution significantly.Based on this,a colorimetric method that can identify His enantiomers is established,and this method is used to determine the His enantiomeric excess(ee),which can be quantitatively determined from-100%to+100%of the D-His ee.In addition,the chiral recognition ability of AuRD for 19 other ?-amino acids was also investigated.Through a series of experiments,the mechanism of AuRD chiral recognition was explored.This study shows that AuRD is an effective chiral recognition probe,which can be used to detect chiral molecules simply and quickly by colorimetry.2.Research on the Chiral Recognition Performance of silver nanoprismsIn this paper,we synthesized citric acid-coated silver nanoprisms(AgNPRs)and characterized their structure.Taking Tyrosine(Tyr)as an example,the chiral recognition performance of AgNPRs modified with no chiral reagents was studied.The experimental results show that L-Tyr can agglomerate AgNPRs,and the color of the solution changes from blue to colorless,while D-Tyr cannot change the color of the solution.On this basis,this paper has studied in detail the recognition mechanism of AgNPRs chiral recognition of tyrosine enantiomers.Using AgNPRs as colorimetric probes,the quantitative detection of enantiomeric excess of Tyr enantiomers was carried out.And the actual samples were used to detect the L-Tyr,the recovery rate of the determination was between 100%and 106%,and the RSD was less than 4%.In addition,the mechanism of AgNPRs chiral recognition of Tyr was discussed.This research proposes a new method of chirality recognition,which is simple,fast,low-cost,and has good application prospects.