Preparation Of Polydopamine Composite Nanoparticles And Their Application In Synergistic Tumor Therapy

Malignant tumor has always been one of the major diseases that threaten human health.The traditional treatment of malignant tumor mainly includes chemotherapy,surgery and radiotherapy,but these methods have inherent limitations such as multiple drug resistance and can not treat cancer thoroughly.With the in-depth study of nanotechnology in cancer treatment,some emerging treatment strategies,such as photothermal therapy,ion interference therapy,nano-catalytic therapy,reactive oxygen therapy and so on,have brought new hope for cancer treatment.Tumor tissue and its microenvironment are more complex,and single therapy is often unable to completely inhibit tumor growth.Therefore,the combination of two or more treatment strategies can not only overcome the limitations of monotherapy.It can also reduce the side effects of nanomaterials in cancer treatment,so as to improve the therapeutic effect.As a material with good biocompatibility,polydopamine can be modified on the surface of almost any particles,which is not only used to improve the surface physical and chemical properties of particles,but also increase the biological applications of particles.In this paper,composite nanoparticles based on polydopamine were synthesized,and ion interference therapy/photothermal therapy synergistic therapy or reactive oxygen therapy/photothermal therapy synergistic therapy were combined to achieve the synergistic effect of the two treatment strategies in the treatment of tumor.The specific research contents are as follows:(1)Polydopamine modified calcium carbonate nanoparticles are used for tumor ion interference/photothermal synergistic therapy: in this chapter,polydopamine modified calcium carbonate nanoparticles(Ca CO3-PDA@PDA NPs)were successfully prepared by gas diffusion method and dopamine hydrochloride self-polymerization method.Through the in vitro degradation of Ca CO3-PDA@PDA NPs under different p H conditions and the photothermal performance test under the condition of nearinfrared laser irradiation,it is concluded that the release of excess calcium ions and good photothermal properties of Ca CO3-PDA@PDA NPs under acidic conditions.Taking cervical cancer cells(He La cells)as cell model,by exploring the efficacy of Ca CO3-PDA@PDA NPs in tumor ion interference therapy,photothermal therapy and ion interference/photothermal synergistic therapy,it is concluded that Ca CO3-PDA@PDA NPs is the best in ion interference/photothermal synergistic therapy.Zebrafish embryo was used as an animal model to demonstrate the in vivo biological safety of low concentration of Ca CO3-PDA@PDA NPs.The above results indicate the application potential of polydopamine modified calcium carbonate nanoparticles in tumor ion interference/photothermal synergistic therapy.(2)Polydopamine-assisted synthesis of gold-platinum nanoparticles for tumor reactive oxygen species/photothermal synergistic therapy: in this chapter,polydopamine-assisted synthesis of gold-platinum nanoparticles(Au Pt-PDA NPs)was successfully prepared by one-step method.Through the peroxidase activity of Au PtPDA NPs and the photothermal properties under the condition of near infrared laser irradiation,it is concluded that Au Pt-PDA NPs has high peroxidase activity and good photothermal properties.Ovarian fibroblasts(CHO cells)were used as a cell model to demonstrate the biological safety of Au Pt-PDA NPs to normal cells.Taking He La cells as cell model,by exploring the efficacy of Au Pt-PDA NPs in reactive oxygen therapy,photothermal therapy and reactive oxygen/photothermal synergistic therapy,it is concluded that Au Pt-PDA NPs is the best in reactive oxygen/photothermal synergistic therapy.The above results show the application potential of gold-platinum nanoparticles synthesized by polydopamine in tumor reactive oxygen species/photothermal synergistic therapy.