| Metal nanoclusters can be divided into noble metal nanoclusters and non-metallic nanoclusters according to their metal species.However,with the rising cost of raw materials and the demand for the development of multifunctional nanoprobes,the development of multifunctional and low-cost metal nanoclusters has become a research focus in recent years.In this thesis,metallic iron nanoclusters and manganese nanoclusters with good magnetic and fluorescent properties were synthesized by a simple method.And the sensitive detection of environmental pollutants potassium dichromate,copper ions and biological small molecules bilirubin and cytochrome C were achieved respectively.The main research contents are as follows:Chapter 1: The recent development of metal nanoclusters at local and overseas is reviewed.And the concept,properties,synthesis methods,applications and main advantages of transition metal nanoclusters are mainly introduced.Chapter 2: A fluorescent-enhanced(" turn-on ")cation and anion sensor based on fluorescent-magnetic iron nanoclusters has been developed and used for the highly sensitive detection of potassium dichromate and copper ions.After the above two ions are discharged into the environment,they will be deposited in large quantities in crops and accumulated to the human body through the food chain.It not only seriously pollutes the ecological environment,but also endangers people's health and reduces the quality of life.Therefore,quantitative monitoring of such environmental pollutants is very necessary.Therefore,it is very necessary to quantitatively monitor these environmental pollutants.In this work,near infrared fluorescently paramagnetic glutathione stabilized iron nanoclusters(GSH-Fe NCs)were synthesized and developed into a "turn-on" fluorescence sensor for the detection of trace potassium dichromic in watermelon,cucumber,lake water,tap water and mineral water.The detection limit of the method was as low as 16 n M,and the linear range was 450 n M ? 35,000 n M.The quantitative peak recoveries were 91.2 % ? 112.6 %.Copper ions can also be detected in tap water and tomato.The detection limit is as low as 8 n M,and the linear range is0.3?M to 35 ?M.The quantitative peak recoveries are 97.1 % ? 104.1 %.Chapter 3: Using the pharmacological effects of tiopronin on bilirubin as an entry point,tiopronin was designed as a template for the synthesis of tiopronin-stabilized iron nanoclusters.This work takes the specific binding of the drug molecule tiopronin to the target bilirubin as an entry point.Tiopronin-stabilized iron nanoclusters were synthesized by designing tiopronin as a template for probes.The linear range of this method is wide,ranging from 0.5 ?M to 200 ?M,and the detection limit is as low as 50 n M.At the same time,the method has been successfully applied to the detection of BR concentration in human serum samples,and the recovery rate of standard addition has obtained satisfactory results,with specific results ranging from 88.8 % ? 104.2 %.Chapter 4: Following the classical template of metal nanoclusters,glutathione,and adding inorganic manganese sources.Manganese ions were reduced to manganese atoms with zero-valence using ascorbic acid as reducing agent.And synthesizing by water bath method,therefor a blue-emitting GSH-Mn NCs probe was prepared.Based on the static quenching mechanism that the two can form a ground-state complex,cytochrome C can quench the fluorescence signal of GSH-Mn NCs at 438 nm.Thus,this method was employed for the highly sensitive detection of cytochrome C.The specificity of this probe recognition is very strong,and it has been successfully used for the detection of cytochrome C concentration in human serum.Chapter 5: Summarizes the results of the analysis and testing of the three fluorescence detectors in this paper,and predicts their later flourishing development. |
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