| Part ?:Characteristics of microcirculation dysfunction in early stage of hypertensive ratsObjects:To study the changes in the morphological and functional characteristics of the peripheral ear microcirculation in early hypertensive rats.Methods:The rats aged 8 weeks were divided into WKY group(n=6)and SHR group(n=6).Blood perfusion,blood flow velocity,blood cell aggregation and microcirculation morphology and function of the rats were monitored by the laser Doppler detection system and laser microcirculation detection system.Results:The results of laser Doppler quantitative detection showed that the peripheral microcirculation blood perfusion of the SHR group was significantly lower than that of the control WKY group(P=0.047);the average blood flow velocity of the peripheral microcirculation of the SHR group was lower than that of the control WKY group(P=0.028).The results of laser microcirculation morphology and function test showed that the tortuosity of the peripheral capillaries in the SHR group was reduced(P=0.153),the density of functional capillaries was significantly reduced(P<0.001),and the total length of capillaries of different diameters was significantly reduced,with a diameter of 30-The total length of capillaries of 50 ?m decreased most significantly(P=0.033).Conclusions:Microcirculation dysfunction has appeared in the early stage of the onset of essential hypertension,which is manifested by a significant reduction in the density of effective functional capillaries and a significant reduction in the total length of functional capillaries of different diameters.It suggests that there are peripheral microcirculation and microvascular dysfunction in the early stage of hypertension,that is,peripheral microcirculation dysfunction is an important cause of hypertension.Part ?:Correlation between blood pressure and microcirculation dysfunction in early hypertensive ratsObjects:Through correlation analysis of blood pressure and microcirculation functional characteristics of early hypertensive rats,explore the correlation between blood pressure and microcirculation dysfunction in early hypertensive rats.Methods:Using laser Doppler blood flow imaging and quantitative detection instrument and laser microcirculation morphology and function analyzer to obtain blood flow perfusion,blood flow velocity,blood cell aggregation,functional capillaries density,functional microvascular flow and microvascular manifolds of different diameter ranges Length,the correlation analysis between blood pressure and all measurement indicators through Pearson correlation analysis software.Results:The correlation analysis between the laser Doppler quantitative detection index results and the laser microcirculation morphology and function detection results showed that the average blood flow velocity was found to be significantly reduced in the SHR group.After wavelet transformation,the blood flow velocity of different sources was comparable to the average blood flow velocity.Consistent,the WKY group is higher than the SHR group.Among them,it was found that the average blood flow velocity was significantly negatively correlated with heart rate,systolic blood pressure,average arterial pressure,and diastolic blood pressure(r=-0.573,-0.662,-0.72,-0.69,P<0.05 or P<0.01),and was related to blood perfusion There was a significant positive correlation(0.868,P<0.01).The average blood cell aggregation was found to be elevated in the SHR group.After wavelet analysis,the difference between the source of cardiac activity and the source of respiratory activity was the most significant(P=0.036,P=0.038).In the correlation analysis,it was found that the heart-derived blood cell aggregation was significantly positively correlated with heart rate,systolic blood pressure,mean arterial pressure,diastolic blood pressure,and pulse pressure difference(r=0.604,0.637,0.678,0.619,0.538,P<0.01),which was significantly negatively correlated with blood perfusion,average blood flow velocity,blood flow velocity derived from muscle activity and blood flow velocity derived from nerve sources(r=-0.691,-0.925?-0.577,-0.653,P<0.01).The degree of blood cell aggregation derived from respiratory activity was significantly positively correlated with heart rate,systolic blood pressure,mean arterial pressure,diastolic blood pressure,pulse pressure difference,and heart-derived blood cell aggregation(r=0.641,0.644,0.653,0.592,0.587,0.959,P<0.05 or P<0.01),which was significantly negatively correlated with average blood flow velocity and blood flow velocity from nerve sources(r=-0.84,-0.539,P<0.05).Capillaries density was significantly negatively correlated with heart rate,systolic blood pressure,mean arterial pressure,diastolic blood pressure,pulse pressure difference,heart-derived blood cell aggregation,and respiratory activity-derived blood cell aggregation(r=-0.864,-0.91,-0.914,-0.865,-0.772,-0.738,-0.79,P<0.01),which was significantly positively correlated with blood perfusion,average blood flow velocity,and microvascular tortuosity(r=0.785,0.754,0.723,P<0.01).Conclusions:Microcirculation dysfunction has appeared in the early onset of essential hypertension arid is significantly correlated with the increase in blood pressure,suggesting that early peripheral microcirculation and microvascular dysfunction is closely related to the increase in blood pressure.Part ?:Characteristics of microcirculation dysfunction in early stage of hypertensive ratsObjects:To study the effect of exosomes on the morphological and functional characteristics of the ear marginal microcirculation in early hypertensive rats.Methods:Use cultured endothelial cells(HUVECs)to collect the supernatant,extract the exosomes from the culture supernatant,and use the BCA protein concentration detection method to detect the concentration after obtaining the exosomes.The rats were divided into WKY group,SHR control SHR-Baseline group(SHR-B),SHR normal exosomes SHR-Cexo group(SHR-Cexo)(n=6),by the method of tail vein injection of exosomes.Carry out laser Doppler detection system and CapiScope CAM1 laser microcirculation function and shape analyzer(CapiScope CAM1 Capillary Anemometer)detection.Observe the blood perfusion,blood flow velocity,blood cell aggregation and functional microcirculation of the blood vessels in the ear margin of the rats at 0 h,5 h and 24 h.Results:After the injection of normal exosomes,the blood perfusion and average blood flow velocity increased significantly.After wavelet transform analysis,the blood flow velocity of endothelial origin increased significantly,and the degree of blood cell aggregation decreased to varying degrees,At the same time,the total length of capillaries in different diameter ranges of rats in the SHR-Cexo group,especially the total length of capillaries with a diameter of less than 50 ?m,the tortuosity of capillaries and the density of functional capillaries also increased significantly(P<0.05).Conclusions:Exosomes derived from endothelial cells can effectively improve the function of microcirculation and capillaries in hypertensive rats,suggesting that exosomes derived from normal endothelium have the potential to treat hypertension by improving microcirculation and slowing down the treatment of hypertension. |
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