| [Objective]This experiment was carried out to evaluate the effect of rubbing middle energizer points on regulating gastric motility and study its mechanism on regulating gastric motility.In this study,the intragastric residual rate was used to evaluate the effect of rubbing middle energizer points on promoting the recovery of gastric motility in rats with functional dyspepsia(FD).The expression of related proteins was detected by immunohistochemistry and Western blot to explore whether the mechanism of rubbing the middle energizer points on promoting the recovery of gastric motility is related to regulating SCF/c-kit pathway and affecting the number of Cajal interstitial cells(ICC).[Methods]Thirty six rats were randomly divided into blank group,model group,western medicine group,acupoint pressing group,acupoint blocking group and model blocking group,with 6 rats in each group.Except for the blank group,the other five groups were used to establish the FD rat model with mild clipping stimulation on tails and irregular diet,the model lasted for 21 days.From the 12th day of modeling to the end of intervention,the acupoint blocking group and model blocking group were intraperitoneally injected with 0.2 mg/kg of c-kit monoclonal antibody(ACK2),once every other day.After successful modeling,each group was intervened accordingly.In the acupoint pressing group and the acupoint blocking group,Lanmen and Jianli were selected and rubbed,the depth is measured by touching the obvious pulsation of abdominal aorta,the frequency is 25～35 times/min.Each point was stimulated for 6 minutes,12 minutes in total.10 mL/kg physiological saline was administered by gavage,once a day.The western medicine group was given 10 mL/kg mosapride solution(calculated according to the clinical dosage and body shape coefficient formula of rats and human,and diluted into 0.137 g/L suspension)by gavage,grabbed for 12 minutes,once a day.The blank group,model group and model blocking group were grabbed for 12 minutes,and intragastric administration of 10 mL/kg physiological saline,once a day.The intervention lasted for 14 days.After the intervention,the intragastric residual rate was measured,antral tissues were taken.The IOD value and positive area ratio of ANO1 were detected by immunohistochemistry.The protein expressions of c-kit,SCF and PI3K were detected by Western blot.[Results]Intragastric residual rate:the model group was significantly higher than that of the blank group(P<0.05),the acupoint pressing group and western medicine group were significantly lower than the model group(P<0.05);there was no significant difference between acupoint pressing group and western medicine group and the blank group(P>0.05);there was no significant difference between the acupoint pressing group and the western medicine group(P>0.05).Compared with the blank group,the acupoint blocking group and model blocking group were significantly higher(P<0.05);there was no significant difference between the acupoint blocking group and the model blocking group and the model group(P>0.05);the acupoint blocking group and model blocking group were significantly higher than the acupoint pressing group(P<0.05);there was no significant difference between the acupoint blocking group and the model blocking group(P>0.05).The ratio of IOD and positive area of ANO1:the change trend of IOD value and positive area ratio in each group was consistent.The model group was significantly lower than that of the blank group(P<0.05),the acupoint pointing group and western medicine group were significantly higher than the model group(P<0.05);there was no significant difference between acupoint pressing group and the western medicine group and the blank group(P>0.05);there was no significant difference between the acupoint pressing group and the western medicine group(P>0.05).Compared with the blank group,the acupoint blocking group and model blocking group were significantly lower(P<0.05);there was no significant difference between the acupoint blocking group and the model blocking group and the model group(P>0.05);compared with the acupoint pressing group,the acupoint blocking group and the model blocking group were significantly lower(P<0.05);there was no significant difference between the acupoint blocking group and the model blocking group(P>0.05).The expression of PI3K:the model group was significantly lower than the blank group(P<0.05);the acupoint pressing group and western medicine group were significantly higher than the model group(P<0.05);there was no significant difference between the acupoint pressing group and the western medicine group and the blank group(P>0.05);there was no significant difference between the acupoint pressing group and the western medicine group(P>0.05).Compared with the blank group,the acupoint blocking group and model blocking group were significantly lower(P<0.05);there was no significant difference between the acupoint blocking group and the model blocking group and the model group(P>0.05);compared with the acupoint pressing group,the acupoint blocking group and model blocking group were significantly lower(P<0.05);there was no significant difference between the acupoint blocking group and the model blocking group(P>0.05).The expression of c-kit:the model group was significantly lower than the blank group(P<0.05);compared with the model group,the acupoint pressing group and western medicine group were significantly higher(P<0.05);there was no significant difference between the acupoint pressing group and the western medicine group and the blank group(P>0.05);there was no significant difference between the acupoint pressing group and the western medicine group(P>0.05).Compared with the blank group,the acupoint blocking group and model blocking group were significantly lower(P<0.05);there was no significant difference between the acupoint blocking group and the model blocking group and the model group(P>0.05);compared with the acupoint pressing group,the acupoint blocking group and model blocking group were significantly lower(P<0.05);there was no significant difference between the acupoint blocking group and the model blocking group(P>0.05).The expression of SCF:the model group was significantly lower than the blank group(P<0.05);compared with the model group,the acupoint pressing group and western medicine group were significantly higher(P<0.05);there was no significant difference between the acupoint pressing group and the western medicine group and the blank group(P>0.05);there was no significant difference between the acupoint pressing group and the western medicine group(P>0.05).Compared with the blank group,the acupoint blocking group and model blocking group were significantly lower(P<0.05).Compared with the model group,the acupoint blocking group was significantly higher(P<0.05),there was no significant difference between the model blocking group and the model group(P>0.05).Compared with the acupoint pressing group,the model blocking group was significantly lower(P<0.05),there was no significant difference between the acupoint blocking group and the acupoint pressing group(P>0.05).The acupoint blocking group was significantly higher than the model blocking group(P<0.05).[Conclusion]Rubbing on the middle energizer points can effectively promote gastric emptying and gastric motility recovery in FD rats,and the curative effect is equivalent to mosapride.The middle energizer points can up regulate the expression of SCF,c-kit and PI3K,promote the number of ICC to return to normal,the effect is similar to mosapride.The mechanism of rubbing the middle energizer points promoting gastric motility recovery is related to up regulating SCF/c-kit pathway and increasing the number of ICC.In addition,the number of ICC may be increased through other ways by rubbing the middle energizer acupoints to promote the recovery of gastric motility. |
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