The Diversity Of Cell Types In Basal Ganglia During Early Developmental Stage

GABAergic inhibitory neurons are crucial in maintaining the stability of neural circuits through synaptic inhibition in the human brain for possess normal activities.Because of the size and complexity of the human brain,it is a challenge to reveal the rich diversity of interneurons.With the development of research tools,high-throughput sequencing is widely used in embryonic development research.To decipher the origin and molecular features of interneurons,we here have captured diverse subtypes of interneuron progenitors and precursors in the early developmental stage of human fetal basal ganglia by conducting single-cell RNA-sequencing.We focus on the distinct subtypes of progenitor and interneuron precursors,and we have found that progenitor possess two different developmental stage which is driven by a string of genes.Trajectory analysis indicated that interneuron precursors derived from the medial and lateral ganglionic eminence(MGE and LGE)development into three and two types of interneuron precursors separately.Besides,we choose autism spectrum disorders(ASD)high risk genes from dataset named SFARI,and clarify these genes depended on their express patterns in subcluster of progenitors and interneuron precursors.This work reveals the origination and diversification of interneurons,which provide insight into the incentive of the diversification of mature cortex interneurons.