Research On Biological Toxicity Monitoring Technology Of Seawater Based On Bivalve Biological Behavior Reaction

Biological effect is an important basis for evaluating water pollution.Water pollution can be effectively monitored by monitoring the changes in the number of biological community,in vivo cell or in vitro behavioral response.At present,there are a variety of biological monitoring methods,but most of them need to collect samples for analysis and processing under laboratory conditions,which has disadvantages such as cumbersome operation,high cost and inability to conduct in-situ monitoring.With the improvement of national attention to marine environment,the existing physical and chemical analysis methods can no longer meet the needs of marine environment comprehensive monitoring.It is of great significance for marine ecological environment protection in China to develop new marine biological monitoring technology and equipment to realize in-situ and real-time monitoring of marine biological comprehensive toxicity.Based on the comprehensive analysis of the existing biological monitoring technologies,this thesis adopted the Marine biological toxicity monitoring method based on bivalve biological behavior reaction,which solved the problem that the existing technologies such as microbial community monitoring method,luminescent bacteria method,fish analysis method and algae fluorescence analysis method could not realize real-time and in-situ Marine environmental monitoring.Based on the characteristics of bivalve opening and closing of scallop Chlamys farreri,a bivalve biotoxicity monitoring device was developed.The monitoring device includes Hall induction module,power module,data acquisition module and communication module.Aiming at the lack of high-precision calibration method for bivalve biobehavior monitoring sensor probe,this paper studied and designed a calibration device for bivalve biobehavior monitoring sensor probe,which is easy to calibrate experiment and can complete high-precision calibration in a short calibration period.According to the electric potential difference between the linear Hall element and the magnet in the device,the actual distance of scallop double shell tensioning was calculated,and the time and distance of scallop double shell tensioning were monitored.In this paper,the seawater biological toxicity monitoring technology based on bivalve biobehavior reaction can simultaneously collect 8 channels of data with sampling interval of 100 ms,sampling accuracy of 1m V,and monitoring accuracy of tension distance of 0.1mm,realizing multi-channel,high-frequency and high-precision real-time monitoring of seawater biological activity.In this thesis,scallop Chlamys farreri was used as the monitoring object,and the relevant verification experiments were carried out in the biological laboratory,and the results of the experiments were analyzed.The biological activity algorithm was used to analyze the opening and closing distance data of the scallop Chlamys farreri to judge its physiological behavior and activity,so as to evaluate the water quality.The test results show that the seawater biological toxicity monitoring device designed in this paper can achieve the purpose of monitoring the double-hull opening and closing distance,realize real-time monitoring of seawater quality,and provide a basis for early warning of marine water pollution.