| Tris(1,3-dichloro-2-propyl)phosphate(TDCIPP)is one of the most widely used organophosphorus flame retardants with high detection rates in environmental media and organisms.Previous studies have shown that TDCIPP can cause embryonic developmental toxicity in zebrafish.In this study,the effects of TDCIPP on zebrafish embryo were investigated by morphological,histopathological and behavioral methods,and the effects of TDCIPP on zebrafish embryo transcription levels were further investigated by transcriptome sequencing technology.The main results are as follows:1)Exposure to 1000 μg/L TDCIPP for 120 h can cause developmental toxicity in zebrafish embryos,mainly manifested as abnormal embryo development trajectory,decreased hatchability and survival rate,increased malformation rate(pericardial edema,trunk curvature and caudal fin loss),abnormal heart rate,growth retardation and reduced swimming ability.2)TDCIPP exposure at 1000 μg/L can inhibit transcriptional activation in MBT stage of zebrafish embryos,increase the number of cells and produce irregular stacking of blastomes,gens related to encode nuclear receptors(nfil3,nr2f6 b,nr1d2a,cited1,foxa1,gsc)and growth factors(grtp1b,pdgfbb,eps15l1 a,tgfbr3,gas2l3)disorderly express.3)TDCIPP exposure decreased the motility of blastomeres and increased the height of blastomere’s height of 5.25 hpf embryos,increased migration distance of 8hpf and 10 hpf blastomere cells,and delayed epiboly development.This might be associated with abnormal expression of genes(lama4,cdh5,fgf3,slc19a1,slc22a21)on the membrane surface that regulate intercellular signaling,adhesion,and material transport.4)TDCIPP exposure can lead to abnormal differentiation of dorsoventral body axis in zebrafish embryos,which may be related to the down-regulation of Wnt pathway related genes(gsk-3β,dvl3)and up-regulated expression of organizer gene gsc. |
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