Functional Analysis Of The Arabidopsis Thaliana Transcription Factor COFU

The known studies have shown that COFU(CO-FACTOR WITH FVE),as a newly discovered gene in Arabidopsis thaliana,can interact with FVE(FLOWERING LOCUS VE),hence it has been named as co-factor with FVE.FVE is one of the component in Pc G(Ploycomb group)family number of PRC2(Polycomb Repressive complex 2).However,the main functions and principles of COFU are still unknown.In this experiment,the function and molecular mechanism of COFU in leaf serration development,vegetative phase change,floral transition and lateral root development were preliminarily explored by COFU deletion mutant cofu-and wild-type Col-0.The contents and results are as follows:1.Through the comparative analysis of the leaf phenotype in cofu-and Col-0,it can be seen that the serration degree and number of cofu-are significantly greater than those of wild types.The q RT-PCR and Ch IP-PCR detection results show that the expression levels of CUC2is significantly up regulated in cofu-,the level of H3K27me~3 is significantly reduced at the CUC2 locus of cofu-.The serration degree and number of cuc2-3 cofu-double mutant is reduced,the mutation has been partly rescued.A series of studies have shown that COFU inhibits the expression of CUC2 by introducing H3K27me~3 markers at the CUC2 locus P2,P3position,thereby represses the leaf serration development.2.Based on the statistical analysis of seed germination rate,true leaf generation rate,abaxial trichomes distribution and generation rate,number and leaf length of rosette leaves and flowering time,it was concluded that compared with Col-0,there was no obvious difference in the time point of cofu-in beginning vegetative growth,but the vegetative phase change and floral transition was earlier.Physiological treatment statistics and q RT-PCR show that COFU mainly delays the VPC and floral transition through FT in the photoperiod pathway and MIR156A-SPL3 in the age pathway,which is not related to gibberellin,autonomous and vernalization pathway.3.Based on the lateral root growth phenotype,q RT-PCR and Ch IP-PCR,GUS staining shows that COFU can directly inhibit the downstream target gene SPL3 by mi R156-independent pathway that introducing the inhibitory chromatin marker H3K27me~3 into the P2site of SPL3 locus.So COFU can promote the lateral roots development.The GUS staining of SPL3 mainly concerntrated in the area of newly forming LRPs,emerging lateral roots and mature lateral roots.Loss of COFU results in the amplification of expression level and region of SPL3::GUS in root.