Research And Development Of Lactic Acid Bacteria Antimicrobial Peptide Database

Lactic acid bacteria are a group of bacteria that use carbohydrates to ferment and form lactic acid.Lactic acid bacteria antimicrobial peptide is a kind of active polypeptide or protein produced in the metabolic process of lactic acid bacteria,which can kill or inhibit many kinds of pathogenic bacteria,and is a natural food preservative and anti-infective drug.Lactic acid bacteria antimicrobial peptides have wide application prospects in food and bio-medicine industry.Studying the sequence structure of antimicrobial peptides of lactic acid bacteria can promote the research of its antibacterial mechanism,but there is no special database of antimicrobial peptides of lactic acid bacteria to store the sequence and related information for researchers in the field.Based on this background,more than 20 public databases of antimicrobial peptides were collected by using 30 key words of lactic acid bacteria,and the first Database of Lactic acid bacteria antimicrobial peptides(DLABAMP)was constructed with Springboot + VUE + MySQL Framework.The website is: http://www.dong-group.cn/database/dlabamp#/.The database contains 1622 lactic acid bacteria antimicrobial peptide records,each of which contains 21 different fields.The website has browsing,search,statistic,analysis,downloading,prediction,sequence alignment function and so on.Record details such as sequence,sequence length,name,family,activity,description,etc.can be obtained by searching or browsing links.Users can retrieve the database simply by the specific field,and can also retrieve the high-level data by the combination of the key words such as sequence,length,source,peptide name and so on.Users can also download records of all antimicrobial peptides they are interested in after searching or browsing operation.In order to show the overall view of the database,the statistical function analyzes the database structure from different perspectives,including amino acid percentage,distribution of peptide length,family,source,activity and so on.After user enters or uploads the amino acid sequence of the suspected lactobacillus antimicrobial peptides,our website can predict the probability of lactobacillus antimicrobial peptides in batches and visualize the prediction results.The platform also incorporates a bioinformatics sequence alignment tool that align user-uploaded sequences of suspected antimicrobial peptides from lactic acid bacteria with sequences in a database using a pairwise or multiple sequence alignment algorithm,the sequence similarity of antimicrobial peptides from lactic acid bacteria will be calculated,and the homology between the sequences will be distinguished by the sequence similarity,and the evolutionary relationship between the sequences will be inferred.In addition,the site allows users to submit data to the site,including amino acid sequence name,source,activity,personal name and contact information,so that dynamic update database content.After tested,the database is safe,reliable,convenient,and can achieve normal function and stable operation.The related information of antimicrobial peptides of lactic acid bacteria stored in this database,the functions of searching,downloading,statistical and sequence alignment and prediction of antimicrobial peptides of lactic acid bacteria provide basic data for the study of the mechanism of antimicrobial peptides against pathogenic bacteria infection and the mechanism of antimicrobial molecules,at the same time,it lays a foundation for the research on the evolution relationship and practical application of antimicrobial peptides from lactic acid bacteria.