Diffuse Reflectance Measurement And Wide-Field 3D Imaging Method Of Biological Tissue Surface

In the context of diffuse reflectance measurement,this has the effect of the shape,position,gesture of the measured tissue to be exactly the same as the reference sample in practice,which may be unacceptable for many applications.The necessary of approaches exist to extract information about the surface shape,location and gesture of the tested tissue to facilitate correction and optimize the measurement of diffuse reflection,which indicates that organizational surface 3D measurement becomes one of the fundamental issues.In this article,the broad-field 3D imaging methods of the biological tissues were researched.We have explored two distinct biological tissue wide-field 3D imaging methods bottom on phase-shifting method and Fourier transform method respectively and coupled them to a set of 3D imaging system.In addition,a zero-frequency diffuse reflectance 3D imaging experiment was produced,respectively on normal apple tissue,oxidized apples tissue and human forearm tissue.The main research contents of the paper are as follows:(1)A diffuse reflectance 3D imaging method based on the phase shift method was researched,which specifically outlined the coding principle of the phase shifting method and the phase unwrapping principle of multi-frequency layering method.In particularly,the errors and the applicable conditions for the layering method were analyzed.Additionally,a mathematical model of 3D imaging system based on positiving strip patterns was implemented.We have researched diffuse reflectance imaging model of sample tissue by phase-shifting method and cultivated the zerofrequency diffuse reflectance 3D imaging simulation system,which are based on the mathematical model of 3D imaging systems based on positiving strip patterns.The adopted system was applied to simulate imaging and evaluate the imaging results.(2)A diffuse reflectance 3D imaging method based on Fourier transform method was researched,which specifically outlined Fourier transform method of the strip analysis principle and the phase unwrapping principle of the multi-frequency heterodyne method.In particularly,the errors and the applicable conditions for the heterodyne method were analyzed.We have proposed and established a mathematical model of a 3D imaging system based on sloping strip patterns and a diffuse reflectance imaging model of sample tissue based on Fourier transform method.The adopted system was applied to simulate imaging and evaluate the imaging results.(3)An actual 3D imaging system and a depth-based zero-frequency diffuse reflectance correction model was been established.To validate the above two 3D diffuse reflectance imaging methods,the actual 3D imaging system has been utilized to demonstrate the efficacy of practical imaging experiments,especially at normal apple tissue,oxidized apples tissue and human forearm skin tissue.