DNA Storage Technology Based On Biological Enzyme

With the development of computer technology,humans have realized the use of magnetic,optical,and electronic information storage media for information storage,while using binary coding schemes to convert information to digital,entering the digital information era.However,today,with the accumulation of massive amounts of information by humans,the speed of generating information is also increasing,which makes it difficult for current storage technologies to cope with the challenges of explosive data growth.DNA storage technology refers to converting information into different sequences of four natural nucleic acid bases(A,T,C,G)in accordance with a pre-designed coding scheme,and then storing it in deoxyribonucleic acid.Compared to traditional storage methods,DNA as information storage media has excellent characteristics such as high storage density and low maintenance costs,which makes DNA storage technology an ideal solution for storing information and is expected to become a new type of information storage method with wide applicability in the future.As a new storage solution,in order to achieve the widespread application of DNA storage technology,problems such as high synthesis cost and low base utilization need to be solved.To address the above issues,this paper proposes a new DNA storage scheme based on biological enzyme reactions,and designs a targeted information encoding scheme to convert digital information into DNA sequences that can be used for actual storage.The main work of this article is as follows:1.Long stranded DNA storage system based on T4 DNA ligase.This system distributes digital information to a large number of DNA information short strands,and then splices the DNA short strands using the principle of base complementary pairing.Through the enzymatic reaction of various biological enzymes such as T4 DNA ligase,a large amount of short stranded DNA that originally formed a composite structure is reacted to generate a complete long stranded DNA sequence,and finally the long stranded DNA is used as a storage medium for information.2.Coding scheme suitable for long stranded DNA storage systems.Aiming at problems such as DNA sequencing errors and DNA molecular base sequence constraints,this paper designs a targeted DNA coding algorithm.Based on the coding scheme,this paper designs a long stranded DNA storage model with complete DNA storage functions.3.Complete biological implementation of a long chain DNA storage system.According to the DNA sequences generated by the coding algorithm,this paper conducted specific biological molecular experiments,which verified the feasibility of the long chain DNA storage system,and obtained the optimal reaction system for realizing the long chain DNA storage system.The long chain DNA storage system constructed in this paper is a new type of DNA storage technology that combines biological enzymatic reactions.Based on experimental analysis of the designed scheme through molecular biology technology,this paper proposes an adaptive coding algorithm to solve the possible problems in the long chain DNA storage system at the algorithmic level.These efforts are a successful combination of biology and computer science.The long chain DNA storage scheme based on biological enzymes not only improves the problems faced by current DNA storage,such as the difficulty of long chain DNA synthesis,high synthesis cost,and low base utilization,but also provides new ideas and solutions for the practical application of DNA storage technology.