Phylogenetic Analysis And Database Construction Of RNA N~6-Methyladenosine Modification Enzymes

N~6-methyladenosine(m~6A)is the most common chemical modification and is involved in a series of biological processes,such as cell development,differentiation,proliferation,and biological rhythm regulation.As a dynamic modification,the process of m~6A modification is regulated by methyltransferases,demethyltransferases,and recognition proteins.However,there are few works focusing on the systematic analysis of m~6A modifcaition enzymes,which hinders our understanding on m~6A modification.By collecting RNA modification enzymes from public databases and literatures,the first database,called RNAWRE,dedicating to storing RNA modification enzymes was constructed,which will be helpful for researches on RNA modifications.Based on RNAWRE,the cross-species comparative genomics analysis of m~6A modification enzymes was performed.By selecting the 7 types of m~6A modifcaition enzymes from animals and plants,the phylogenetic and sequence analysis of them were performed in107 representative animals,plants and fungi.It was found that the motifs and their orders in m~6A recognition proteins and demethyltransferases were distinct in animals,plants and fungi,while their methyltransferases were highly conserved.The results of selection pressure analysis showed that the evolution rates of m~6A regulators were similar in evolutionarily closely related species.The adaptive evolution was found among species,which is obvious for m~6A recognition proteins and demethyltransferases.The results of the colinear analysis indicated that there were evolutionary differences between monocots and dicots plants,and the gene replication mode of m~6A regulators was mainly affected by whole-genome duplication.In addition,m~6A regulators from different species exhibit different codon bias.In conclusion,distinct evolution rates were observed for different types of m~6A regulators.The methyltransferases that play key roles in the process of m~6A modification are the most conserved,while the m~6A recognition proteins and demethyltransferases are more relaxed and exhibit the adaptive evolution.Figure 14;Table 17;Reference 96...