Preparation Of Fluorescent Probes For Detecting Mercury (Ⅱ) And Copper (Ⅱ) Ions

Metal ions play a vital role in many important biological processes,including cell signaling,metabolism,energy production,catalysis,biomineralization and immune response.The pollution of heavy metal ions in water seriously threatens the environment and human health.The development of a simple and inexpensive detection method that can quickly detect metal ions in real time with high sensitivity is very important for environment-analytical and biomedical applications.In this paper,two fluorescent probes RBM and CPB were designed and synthesized for the detection of mercury ions and copper ions with fluorescence"turn-on"and"turn-off",respectively.The recognition properties and processes were studied subsequently through a series of analysis.The results were summarized into the following two parts:A fluorescent chemical probe RBM based on rhodamine was designed and synthesized,and it was characterized by proton nuclear magnetic resonance and mass spectrometry.The probe RBM showed weak fluorescence in CH3CH2OH/H2O solution.After the addition of Hg²⁺ions,RBM had a significant fluorescence enhancement with a peak at 586 nm,while the fluorescence intensity had a good linear relationship with the concentration of Hg²⁺ions.When 20 equivalents of Hg²⁺ions were added,the fluorescence intensity reached about 24times of the initial.The probe RBM can selectively detected Hg²⁺ions in the presence of other metal ions.The probe RBM was successfully applied to detect Hg²⁺ions in Hela cells.A fluorescent chemical probe CPB based on coumarin was designed and synthesized,which was confirmed by proton nuclear magnetic resonance and mass spectrometry.The probe CPB showed strong fluorescence in CH3CN/HEPES solution with a peak at 490 nm.After the addition of Cu²⁺ions,the fluorescence of CPB was significantly quenched while the fluorescence intensity had a good linear relationship with the concentration of Cu²⁺ions.When 20 equivalents of Cu²⁺ions were added,the fluorescence intensity reached about 30%of the initial.The detection of Cu²⁺ions was barely affected in the present of other metal ions.The probe CPB could be used to detect Cu²⁺ions in Hela cells and zebrafish.