Study On The Influence Of Fluorescence Anisotropy Microenvironment Based On Polarization Imaging Quantum Dots

Fluorescence polarization anisotropy is sensitive to temperature and other parameters at the micro/nano scale,and can be used to measure various physical parameters at the micro/nano scale.Fluorescence polarization anisotropy has a wide range of applications in medical ultrasound imaging and biomacromolecule detection.In order to facilitate and efficiently measure fluorescence anisotropy and study the impact of fluorescence anisotropy microenvironment,this paper uses a fluorescence polarization anisotropy detection system based on polarization imaging to study the polarization anisotropy effects of quantum dots fluorescence on microenvironments such as concentration,temperature,nanoparticles,etc.,providing technical support for biomolecular fluorescence testing.The polarization imaging system is used to collect polarized light and polarized fluorescence images during the experimental process.After processing and calculating the polarization images,the required changes in fluorescence polarization anisotropy for the experiment are ultimately obtained.The paper established a fluorescence polarization anisotropy detection system,and selected Cd Se/Zn S quantum dots with good stability and easy operation as the fluorescence solution for experimental research through research and analysis.Then,the fluorescence polarization anisotropy of the solution was obtained through experimental measurement of polarization fluorescence.In the study of the influence of fluorescence polarization anisotropy on microenvironment,firstly,the fluorescence anisotropy of Cd Se/Zn S quantum dots solution with concentrations of1mol/ml,5mol/ml and 10mol/ml respectively was measured,and the influence of concentration on fluorescence polarization anisotropy was analyzed.The experiment found that the average fluorescence anisotropy was 0.1608,0.0880 and 0.0409,respectively.As the concentration increases,the fluorescence anisotropy of the quantum dots solution decreases;By measuring the fluorescence anisotropy of Cd Se/Zn S quantum dots solutions at five different temperatures:0 ℃,20 ℃,40 ℃,60 ℃,and 80 ℃,the average values of fluorescence anisotropy were0.1608,0.0957,0.08580,0.0354,and 0.0296,respectively.It was found that as the temperature increased,the fluorescence anisotropy of the quantum dots solution also decreased;Due to the introduction of some nanomaterials as enhancing agents for fluorescence polarization anisotropy in recent years,significant progress has been made in the study of fluorescence polarization anisotropy.Therefore,by adding gold nanorods,the effect on the anisotropy of fluorescence solution was observed.The experiment showed that after adding gold nanorod solution,the fluorescence anisotropy of quantum dots increased.The results indicate that the combination of polarization imaging system and detection of fluorescence polarization anisotropy can accurately measure fluorescence polarization anisotropy.The fluorescence polarization anisotropy detection system based on polarization imaging is not only easy to operate and safe,but also has advantages such as good stability,fast response speed,small size,low cost,high integration,and little external influence.Therefore,it has good application prospects in fields such as biomolecular detection and medicine.