Construction Of Titanium Dioxide Photocatalytic Fluorescence Sensing Platform For Protein Quantification

Titanium dioxide（TiO₂）nanomaterials are an important photocatalyst that has been widely studied due to its low cost and good performance.In recent years,the applications of TiO₂nanomaterials in the field of analytical sensing have been continuously developed.In this paper,a fluorescence sensing platform has been developed based on the photocatalytic properties of TiO₂,and a high-throughput rapid quantification method that can be used for trace proteins has been successfully constructed on this basis.The details of the study are as follows:1.Titanium dioxide nanosheets（TiO₂-NS）were prepared by the solvothermal method.The structure and properties of the synthesized TiO₂-NS were characterized by transmission electron microscopy（TEM）,UV-Vis absorption spectroscopy,X-ray diffraction,and zeta potential.The results showed that TiO₂-NS is an anatase type with dispersed particles,homogeneous size and good stability,and has good absorption of UV light at wavelengths of 200～300 nm.For the first time,the TiO₂photocatalytic reduction of the non-fluorescent dye Resazurin to the fluorescent product Resorufin was achieved by using tris-（hydroxymethyl）-aminomethane（Tris）as a photogenerated hole scavenger,and a photocatalytic fluorescence sensing platform based on TiO₂concentration was established.2.The non-specific adsorption ability of TiO₂-NS on proteins on the surface of polystyrene 96-well plates was found by a TiO₂photocatalytic fluorescence sensing platform.Taking bovine serum albumin（BSA）as an example,the results showed that the non-specific adsorption of TiO₂-NS on BSA was influenced by protein concentration,salt ions,and surfactant factors.Tween-20 nonionic surfactant was found to significantly enhance the adsorption ability of TiO₂-NS on trace proteins on well plates,and the phosphate buffer concentration and p H were optimized to achieve a sensitive response to trace BSA in the samples.3.Based on the detection and analysis characteristics of the 96-well plate,a novel protein quantification method with ultra-sensitive,high-throughput and rapid detection was established.The method is applicable to the detection of total protein in samples.The quantitative analysis of human serum albumin in artificial urine was used as an example to verify the practical application potential of the method and to provide strong support for the subsequent development of the method.In summary,the research and findings of this paper expand the application fields of TiO₂nanomaterials and provide new ideas for the development of novel protein quantification methods.