Study On The Effect Of Thermophilic Microbial Agent Enhancing The Bio-drying Of Food Waste

Bio-drying is a promising way to reduce,harmless and recycle food waste.However,it still has some problems,such as a long time of microbial colonization and low efficiency in the early stage.In this study,thermophiles were enriched from the sediments of Tengchong Hot Spring to prepare solid thermophilic microbial agents,which were applied to the bio-drying of food waste.Study the effects of inoculation with the thermophilic microbial agent on the bio-drying of food waste,and analyze the change of physicochemical properties and the succession of microbial communities during the process.To provide theoretical basis and technical support for the extension and application of thermophilic microbial to enhance the bio-drying of food waste.The main conclusions are as follows:(1)The optimum fermentation medium for thermophiles in hot spring sediments is the CYS medium.The thermophilic microbial community comprises Symbiobacterium(86.40%)and Acinetobacter(6.79%),etc.,with diatomite as a carrier and trehalose as a protective agent,the viable count of solid thermophilic microbial agents prepared after vacuum freeze-drying was 2.1×10~9 CFU/g.(2)Inoculation of thermophilic microbial agents increased the maximum temperature,temperature integration index and water removal rate(TB 55.7℃,219.5℃and 86.11%,CK 52.1℃,159.1℃and 56.02%).Correlation analysis showed that temperature was an important factor affecting bio-drying.(3)Inoculation with a thermophilic microbial agent can significantly improve the alpha diversity of the bacterial community and change the community structure.Thermophiles could rapidly colonize the material with a maximum abundance of 5.13%.The moisture content,temperature,electrical conductivity,total nitrogen,crude fat and crude protein were significantly correlated to the changes in the bacterial community(p<0.05).Compared with the control group,the interaction between species in the bacterial community of the experimental group presents a more complex network structure.(4)Inoculation with a thermophilic microbial agent had little effect on the alpha diversity and composition of the fungal community,and pH(29.12%),water content(13.94%)and electrical conductivity(13.58%)had a relatively high explanatory rate for the changes in fungal community structure.The interdomain interaction network structure of the experimental group was more complex than that of the control group.The interaction between bacteria and fungi in the control group was more closely in the interdomain network.